PLZF Antibody AF2944 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPLZF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2944
NCBI Gene AliasesPLZF, ZNF145
ApplicationsIHC-F,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry
Conjugate/TagUnconjugated
IsotypeIgG
TargetPLZF
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Namezinc finger and BTB domain containing 16
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human PLZF, Met1-Gln254, Accession # Q05516
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human PLZF in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Undifferentiated spermatogonia modulate their behavior via the expression of basement membrane protein laminin†Biology of ReproductionApril 13, 2026Yusuke Kawabe, Saya Yamada, Yuichi Shima, Kentaro Tanemura, Shosei Yoshida, Kenshiro HaraUSA: AF560), anti-Lam1 + 2 (1:500 dilution; Abcam: ab7463), anti-PLZF (1:1000 dilution; R&D Systems: AF2944), anti-Rarg (1:500 dilution; Cell Signaling Technology, Danvers, MA, USA: #8965), anti-PH3 (1:500 dilution;

(2) Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular OrganoidsAdvanced ScienceJanuary 1, 2026Jiachen Sun, Lifa Zhu, Yutong Li, Jinyan Tang, Tao Zhang, Hengjia Zhang, Wanchu Wang, Lufan Li, Shuhui Bian, Xin WuMillipore, AB5535), TRA98 (1:2000, Abcam, ab82527), DDX4 (1:500, Abcam, ab13840), PLZF (1:400, R&D Systems, AF2944), STRA8 (1:200, Abcam, ab308124), c‐KIT (1:200, R&D Systems, AF1356), SYCP3 (1:400, Abcam, ab97672),

(3) A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular NicheAdvanced ScienceJuly 28, 2025Yu Xia, Xiaoxuan Zhang, Bohang Zhang, Minhui Lu, Qing Cheng, Li Liu, Lifang Shi, Yiqiang Cui, Dingdong Chen, Laihua Li, et al.Ab26350), ZO1 (1:200; Thermo Fisher, 617300), GFRα1 (1:500; R&D systems, AF560), PLZF (1:500; R&D systems, AF2944), CKIT (1:200; R&D systems, AF1356), KI67 (1:500, Abcam, Ab16667), SYCP3 (1:200, Abcam, ab97672). The

(4) The addition of 5-aminolevulinic acid to HBSS protects testis grafts during hypothermic transportation: a novel preservation strategyAsian journal of andrologyJuly 1, 2025Ma MH, Chen PG, He JX, Chen HC, Xu ZH, Lv LY, Li YQ, Liang XY, Liu GH.Primary antibody,Dilution,Catalog number,Manufacturer,Experiment Anti-PLZF,1:100,AF2944,R&D Systems,Immunofluorescence Anti-UCHL1,1:100,13179S,Cell Signaling Technology, Anti-cleaved caspase-3,1:100,9664T,Cell Signaling Technology

(5) BRCA1 preserves genome integrity during the formation of undifferentiated spermatogoniaEMBO ReportsMay 28, 2025Peng Li, Licun Song, Longfei Ma, Chunsheng Han, Lejun Li, Lin-Yu Lu, Yidan Liuanti-GCNA,Abcam,ab82527 Rabbit anti-BRCA1,"Wu et al, 2009",N/A Rabbit anti-PLZF,Santa Cruz,sc22839 Goat anti-PLZF,R&D Systems,AF2944 Goat anti-KIT,R&D Systems,AF1356 Goat anti-GFRα1,R&D Systems,AF560 Goat anti-SOX3,R&D Systems,AF2569 Rabbit

(6) The retrotransposon-derived capsid genes PNMA1 and PNMA4 maintain reproductive capacityNature AgingApril 22, 2025Thomas W. P. Wood, William S. Henriques, Harrison B. Cullen, Mayra Romero, Cecilia S. Blengini, Shreya Sarathy, Julia Sorkin, Hilina Bekele, Chen Jin, Seungsoo Kim, et al.incubated with goat anti-PLZF primary antibody at 1:500 dilution (Human PLZF Antibody; R&D Systems, AF2944) at 4 °C overnight. Stained slides were blocked by 3 × 10-min washes in blocking buffer, incubated with

(7) Age‐Dependent Clonal Expansion of Non–Sperm‐Forming Spermatogonial Stem Cells in Mouse TestesAging CellFebruary 22, 2025Terumichi Kawahara, Shinnosuke Suzuki, Toshinori Nakagawa, Yuki Kamo, Miki Kanouchi, Miyako Fujita, Maki Hattori, Atsuko Suzuki, Kentaro Tanemura, Shosei Yoshida, et al.Cell Signaling Technology), anti‐EGR4 (1:50 dilution; ab198197, Abcam), and anti‐PLZF (1:1000 dilution; AF2944, R&D Systems, Minneapolis, MN, USA) antibodies at 4°C for 12 h. The reaction was visualized with donkey

(8) Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factorCommun BiolJanuary 30, 2025Taichi Sugawara, Kayoko Sonoda, Nattapran Chompusri, Kazuhiro Noguchi, Seiji Okada, Mikio Furuse, Tomohiko Wakayamarabbit polyclonal anti-PLZF (1:500; #HPA001499; Sigma–Aldrich); goat polyclonal anti-PLZF (1:300; #AF2944; R&D Systems); goat polyclonal anti-LIN28A (1:300; #AF3757; R&D Systems); goat polyclonal anti-KIT (1:300;

(9) A behind-the-scenes role of BDNF in the survival and differentiation of spermatogoniaAsian Journal of AndrologyJanuary 1, 2025Shin-ichi Tomizawa, Kazushige Kuroha, Michio Ono, Kuniko Nakajima, Kazuyuki Ohboreported previously.19 The following antibodies were used with indicated dilutions: anti-PLZF (1:200; AF2944; R&D systems, Minneapolis, MN, USA), anti-KIT (1:1500; ab112177; Abcam, Cambridge, MA, USA), Alexa 488

(10) AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humansCell death & diseaseJuly 12, 2024Houston BJ, Nguyen J, Merriner DJ, O'Connor AE, Lopes AM, Nagirnaja L, Friedrich C, Kliesch S, Tüttelmann F, Aston KI, et al.µg/ml, Abcam ab13840), the undifferentiated spermatogonia markers PLZF (day 5–35; 0.6 µg/ml, R&D Systems AF2944) and FOXO1 (day 7; 1 µg/ml, Cell Signaling Technology #2880), and differentiating spermatogonia marker

(11) Inter- and trans-generational impacts of real-world PM(2.5) exposure on male-specific primary hypogonadismCell discoveryApril 23, 2024Wei X, Zhang Z, Gu Y, Zhang R, Huang J, Li F, He Y, Lu S, Wu Y, Zeng W, et al.following primary antibodies: rabbit anti-SOX9 (Millipore, Cat# AB5535), goat anti-PLZF (R&D Systems, Cat# AF2944), rabbit anti-CYP17A1(Abcam, Cat# ab125022), rabbit anti-HSD3B1(Invitrogen, Cat# MA5-42697), rabbit

(12) Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesisJournal of biomedical researchDecember 8, 2023Yao Y, Shi B, Zhang X, Wang X, Li S, Yao Y, Guo Y, Chen D, Wang B, Yuan Y, et al.(1∶200; ab26350, Abcam), anti-SOX9 (1∶200; AB5535, Millipore, Billerica, MA, USA), anti-PLZF (1∶200; AF2944, R&D Systems, Minneapolis, MN, USA). Secondary antibodies were FITC- or TRITC-conjugated goat anti-mouse

(13) AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humansbioRxivNovember 17, 2023Brendan J. Houston, Joseph Nguyen, D. Jo Merriner, Anne E. O’Connor, Alexandra M. Lopes, Liina Nagirnaja, Corinna Friedrich, Sabine Kliesch, Frank Tüttelmann, Kenneth I. Aston, et al.ab13840), the pro- and undifferentiated spermatogonia marker PLZF (day 5–35; 0.6 μg/ml, R&D Systems AF2944), and dual stained with the transcription factor marker FOXO1 (day 7; 1 μg/ml, Cell Signaling Technology

(14) SRSF2 is required for mRNA splicing during spermatogenesisBMC biologyOctober 23, 2023Lei WL, Du Z, Meng TG, Su R, Li YY, Liu W, Sun SM, Liu MY, Hou Y, Zhang CH, et al.Inc.); MVH (mouse, ab27591; Abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems); SFRS2 polyclonal antibody (rabbit, 20,371–1-AP, Proteintech); SC35 antibody (mouse, S4045,

(15) Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradationNature communicationsAugust 18, 2023Yamanaka S, Furihata H, Yanagihara Y, Taya A, Nagasaka T, Usui M, Nagaoka K, Shoya Y, Nishino K, Yoshida S, et al.

(16) Gene dosage changes in KCTD13 result in penile and testicular anomalies via diminished androgen receptor functionThe FASEB JournalJanuary 26, 2023Abhishek Seth, Armando Rivera, Ahmed Chahdi, In‐Seon Choi, Olga Medina‐Martinez, Shaye Lewis, Marisol O'Neill, Alex Ridgeway, Joshua Moore, Carolina Jorgez, et al.Jose CA), rabbit monoclonal to SOX9 (1:200, Abcam); and goat polyclonal to PLZF for IHC (1:200) (Cat # AF2944, R & D Systems, Minneapolis MN). Primary antibodies were diluted in PBS plus 5% normal serum belonging

(17) SRSF1-mediated alternative splicing is required for spermatogenesisInternational journal of biological sciencesJanuary 1, 2023Lei WL, Li YY, Du Z, Su R, Meng TG, Ning Y, Hou G, Schatten H, Wang ZB, Han Z, et al.Inc.); MVH (mouse, ab27591; Abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems); SFRS1 polyclonal antibody (rabbit, 12929-2-AP, Proteintech); c-Kit antibody (goat, AF1356,

(18) SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicingeLifeNovember 10, 2022Liu W, Lu X, Zhao ZH, Su R, Li QL, Xue Y, Gao Z, Sun SS, Lei WL, Li L, et al.polyclonal antibody (NB110-93598, Novus Biologicals, 1:1,000); goat anti-PLZF polyclonal antibody (AF2944, R&D, 1:1,000); rabbit anti-DDX4/MVH polyclonal antibody (ab13840, Abcam, 1:1,000); rabbit anti-phospho-Histone H2A.X (Ser139/Tyr142

(19) Gene Dosage Changes in KCTD13 Result in Penile and Testicular Anomalies Via Diminished Androgen Receptor FunctionFASEB JNovember 1, 2022Abhishek Seth, Armando Rivera, In-Seon Choi, Olga Medina-Martinez, Shaye Lewis, Marisol O’Neill, Alex Ridgeway, Joshua Moore, Carolina Jorgez, Dolores J. LambJose CA), rabbit monoclonal to SOX9 (1:200, Abcam); and goat polyclonal to PLZF for IHC (1:200) (Cat # AF2944, R & D Systems, Minneapolis MN). Primary antibodies were diluted in PBS plus 5% normal serum belonging

(20) PDCL2 is essential for spermiogenesis and male fertility in miceCell death discoveryOctober 17, 2022Li M, Chen Y, Ou J, Huang J, Zhang X.were as follows: rabbit anti-PDCL2 antibody (1: 5000), goat anti-PLZF antibody (1:500; R&D Systems, AF2944), mouse anti-GATA4 antibody (1:100; Santa Cruz Biotechnology, sc-25310), mouse anti-SYCP3 antibody (1:200;

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Human PLZF Antibody

PLZF Antibody

PLZF Antibody

PLZF Antibody

PLZF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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PLZF Antibody from R&D Systems, a Bio-Techne Brand

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