Immunofluorescence analysis of DOPA Decarboxylase was performed using 70% confluent log phase SH-SY5Y and SK-O-V3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with DOPA Decarboxylase Polyclonal Antibody (Product # PA1-4651) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization for DOPA Decarboxylase in SH-SY5Y cells. Panel e represents merged image of SK-O-V3, which is reported to be low expressing for the same. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.