Legionella Antibody PA1-7227 from Thermo Fisher Scientific

Product Specs
ItemLegionella Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-7227
IsotypeIgG
ClonalityPolyclonal
ReactivityBacteria
ApplicationsImmunocytochemistry
HostRabbit
Quantity1 mL
FormatPBS with 0.1% sodium azide; pH 7.2
Conjugate/TagUnconjugated
TargetLegionella
Concentration5 mg/mL
ImmunogenA whole cell preparation of Legionella pneumophila; ATCC #33152
Storage TemperatureMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles
PurityProtein A

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) LegioTyper: Rapid typing of Legionella pneumophila serogroup 1 by flow-based chemiluminescence sandwich microarray immunoassayAnalytical and Bioanalytical ChemistryNovember 5, 2025C. Bärwinkel, A. Petzold, C. Lück, M. Petzold, M. Seidel(BioRad, Hercules US-CA). As detection and capture antibodies, three polyclonal antibodies were tested: PA1-7227 (Thermo Fisher, Rockford, US-IL); B65051B (Meridian Life Science, Memphis, US-TN); and OBT0943 (BIO-RAD,

(2) Canonical and phosphoribosyl ubiquitination coordinate to stabilize a proteinaceous structure surrounding the Legionella -containing vacuolebioRxivJuly 27, 2025Adriana Steinbach, Chetan Mokkapati, Shaeri Mukherjee(1:50)",Sigma (F1804) HA-HRP,WB (1:1000),Thermo (26183-HRP) L. pneumophila,Opsonization (1:2000),Thermo (PA1-7227) Myc,WB (1:1000),Proteintech (60003-2-Ig) GFP,WB (1:1000),Roche (11814460001) Ubiquitin (P4D1),WB

(3) Legionella uses host Rab GTPases and BAP31 to create a unique ER nicheCell ReportsDecember 24, 2024Attinder Chadha, Yu Yanai, Hiromu Oide, Yuichi Wakana, Hiroki Inoue, Saradindu Saha, Manish Paul, Mitsuo Tagaya, Kohei Arasaki, Shaeri Mukherjeebacteria were diluted in complete media and mixed with a Legionella polyclonal antibody (Invitrogen, Cat #PA1–7227) at 1:1,000 dilution. The mixtures were then incubated for 20 min at room temperature in a rotary mixer.

(4) Cross-family small GTPase ubiquitination by the intracellular pathogen Legionella pneumophilaMolecular biology of the cellMarch 1, 2024Steinbach A, Bhadkamkar V, Jimenez-Morales D, Stevenson E, Jang GM, Krogan NJ, Swaney DL, Mukherjee S.(3933S) HA (HRP conjugate),1:1000 (WB),Thermo (26183-HRP) L. pneumophila,1:2000 (opsonization),Thermo (PA1-7227) Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Alexa Fluor 633),1:500 (IF),Life Technologies

(5) A Legionella toxin exhibits tRNA mimicry and glycosyl transferase activity to target the translation machinery and trigger a ribotoxic stress responseNature Cell BiologyNovember 1, 2023Advait Subramanian, Lan Wang, Tom Moss, Mark Voorhies, Smriti Sangwan, Erica Stevenson, Ernst H. Pulido, Samentha Kwok, Robert J. Chalkley, Kathy H. Li, et al.bacteria were diluted in complete media and mixed with a Legionella polyclonal antibody (Invitrogen, Cat #PA1–7227) at 1:1,000 dilution. The mixtures were then incubated for 20 minutes at room temperature in a rotary

(6) Effector-mediated subversion of proteasome activator (PA)28αβ enhances host defense against Legionella pneumophila under inflammatory and oxidative stress conditionsPLoS pathogensJune 1, 2023Ngwaga T, Chauhan D, Salberg AG, Shames SR.bovine serum albumin (BSA; w/v) in PBS]. Coverslips were stained with 1:1,000 rabbit α-L. pneumophila (Invitrogen, PA17227), 1:2,000 rat α-LAMP1 (Developmental Studies Hybridoma Bank) primary antibodies and 1:500 Alexa 488-conjugated

(7) Intrabacterial Regulation of a Cytotoxic Effector by Its Cognate Metaeffector Promotes Legionella pneumophila VirulencemSphereFebruary 21, 2023Chauhan D, Joseph AM, Shames SR.induce gene expression. Bacteria were opsonized with α-L. pneumophila antibody (1:1,000 [Invitrogen; PA17227]) in DMEM 10% HIFBS supplemented with 1 mM IPTG for 20 min at room temperature (RT) with rotation. Cells

(8) Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectorsLife science allianceDecember 1, 2021Ibe NU, Subramanian A, Mukherjee S.a MOI of 100, 50, 25 or 5. If cells required opsonization, Legionella polyclonal antibody (Cat. no. PA1-7227; Invitrogen) was used at 1:2,000 and incubated for 20 min at room temperature. Immediately after infection,

(9) The Legionella pneumophila Metaeffector Lpg2505 (MesI) Regulates SidI-Mediated Translation Inhibition and Novel Glycosyl Hydrolase ActivityInfection and immunityApril 20, 2020Joseph AM, Pohl AE, Ball TJ, Abram TG, Johnson DK, Geisbrecht BV, Shames SR.IPTG, followed by opsonization by incubation for 30 min with an ␣-L. pneumophila antibody (Invitrogen; PA17227; 1:1,000) at room temperature. The cell culture medium was supplemented with 1 mM IPTG to ensure expression

(10) Immunomagnetic Separation of Microorganisms with Iron Oxide NanoparticlesChemosensorsFebruary 27, 2020Julian A. Thomas, Florian Schnell, Yasmin Kaveh-Baghbaderani, Sonja Berensmeier, Sebastian P. Schwaminger@Epoxy Particle in Antibody Coupling the further text. Specific rabbit anti-Legionella antibodies (PA1-7227) from Thermo Fisher Scientific are immobilized on @Epoxy particles. The coupling is achieved by direct

(11) Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR ScreenCell host & microbeOctober 9, 2019Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, Jimenez-Morales D, Stevenson E, Krogan NJ, et al.the desired amount of time. METHOD DETAILS Antibodies Rabbit polyclonal anti-Legionella antibody (PA1–7227, Thermo Fisher Scientific, 1:2000 dilution) and mouse polyclonal anti-Legionella antibody [gift from

(12) The Legionella effector LtpM is a new type of phosphoinositide-activated glucosyltransferaseThe Journal of biological chemistryFebruary 22, 2019Levanova N, Mattheis C, Carson D, To KN, Jank T, Frankel G, Aktories K, Schroeder GN.Rospert (Freiburg University)" α-Tubulin (DM1A),Mouse,Santa Cruz Biotechnology (Germany) Legionella (PA1-7227),Rabbit,ThermoFisher Scientific (United Kingdom) "HA.11, clone 16B12 (MMs-101P-1000)",Mouse,Cambridge

(13) The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella VirulenceMethods in molecular biology (Clifton, N.J.)January 1, 2019Frankel G, Schroeder GN.

(14) Legionella pneumophila Effector LpdA Is a Palmitoylated Phospholipase D Virulence FactorInfection and immunityOctober 1, 2015Schroeder GN, Aurass P, Oates CV, Tate EW, Hartland EL, Flieger A, Frankel G.Cambridge Bioscience), mouse anti-Myc tag (05-724, Millipore), rabbit anti-Legionella lipopolysaccharide (PA1-7227; Affinity BioReagents), rabbit anti-Giantin (ab24586; Abcam), anti-c-Myc–fluorescein isothiocyanate

(15) A new method to determine in vivo interactomes reveals binding of the Legionella pneumophila effector PieE to multiple rab GTPasesMBioAugust 12, 2014Mousnier A, Schroeder GN, Stoneham CA, So EC, Garnett JA, Yu L, Matthews SJ, Choudhary JS, Hartland EL, Frankel G.LC-MS/MS. 1/500), mouse anti-Myc tag (Millipore 05-724; 1/500), rabbit antiL. pneumophila (Affinity BioReagents PA1-7227; 1/800), rabbit anticalnexin (Stressgen SPA-860F; 1/100), and rabbit antigiantin (Abcam ab24586; 1/500).

(16) LtpD is a novel Legionella pneumophila effector that binds phosphatidylinositol 3-phosphate and inositol monophosphatase IMPA1Infection and immunityNovember 1, 2013Harding CR, Mattheis C, Mousnier A, Oates CV, Hartland EL, Frankel G, Schroeder GN.(F2047-100; Sigma), mouse anti-Myc (05-724; Millipore), rabbit anti-Legionella lipopolysaccharide (LPS; PA17227; Affinity Bioreagents), mouse anti-EEA1 (catalog no. 610457; BD Transduction Laboratories), mouse anti-LAMP2

(17) The Dot/Icm effector SdhA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J miceInfection and immunityJuly 1, 2013Harding CR, Stoneham CA, Schuelein R, Newton H, Oates CV, Hartland EL, Schroeder GN, Frankel G.and permeabilized as previously described (21) and stained with rabbit anti-L. pneumophila antibody (PA1-7227; Affinity BioReagents), donkey antirabbit IgG–Alexa Fluor 488 – conjugated antibody (711-485-152; Jackson ImmunoResearch),

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Legionella Antibody from Thermo Fisher Scientific

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Legionella Antibody from Thermo Fisher Scientific

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