Western blot was performed using Anti-Gamma-aminobutyric acid receptor-associated protein Polyclonal Antibody (Product # PA1-4146) and 13.9 kDa band corresponding to Gamma-aminobutyric acid receptor-associated protein was observed. Chloroquine is known to increase the expression of Gamma-aminobutyric acid receptor-associated protein. Membrane enriched cell extracts (30 µg lysate) of A549 (Lane 1) and A549 treated with chloroquine (50uM/ml for 20 hr) (Lane 2), NIH/3T3 (Lane 3) and NIH/3T3 treated with chloroquine (50uM/ml for 20 hr) (Lane 4), HeLa (Lane 5) and HeLa treated with chloroquine (50uM/ml for 20 hr) (Lane 6), LNCaP (Lane 7), Mouse Brain (Lane 8) and Mouse skeletal muscle (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).