GLUT4 Antibody PA1-1065 from Thermo Fisher Scientific

Product Specs
ItemGLUT4 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-1065
NCBI Gene AliasesGlut4
ClonalityPolyclonal
Quantity100 µg
ApplicationsWestern Blot
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
TargetGLUT4
ReactivityRat
HostRabbit
Conjugate/TagUnconjugated
NCBI Full Gene Namesolute carrier family 2 member 4
Concentration1 mg/mL
ImmunogenSynthetic peptide corresponding to residues E(493) Q E V K P S T E L E Y L G P D E N D(509) of rat GLUT4
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) A melanocortin 4- and glucagon-like peptide 1 receptor multiple agonist for the treatment of diabetes and obesityMetabolismJanuary 1, 2026Emily F. Ashlaw, Clinton T. Elfers, Kylie S. Chichura, Isabella Chavez Miranda, Aelish McGivney, Oleg G. Chepurny, George G. Holz, Ginger Mullins, Laura J. den Hartigh, Yongjun Liu, et al.4–20 % SDS-PAGE, transferred to a nitrocellulose membrane, and probed for rat Glut4 (ThermoFisher #PA1-1065) and total protein (Licor, Revert 700 Total Protein Stain #926-11010). 2.13. In vitro stability in

(2) Divergent Changes in Peak Fat Oxidation and Fat(max) Following 3-Day Dietary Interventions Are Related to Muscle Glycogen Availability in MenScandinavian journal of medicine & science in sportsSeptember 1, 2025Tarry EK, Vestergaard SG, Petersen EA, Olsen MC, Hansen M, Ingersen A, Connor T, Pilegaard H, Dela F, Larsen S, et al.Sigma‐Aldrich), PLIN5 (rabbit polyclonal, NB110‐60509, NovusBiologicals), and GLUT4 (rabbit polyclonal, PA1‐1065, ThermoFisher). Following incubation in primary antibodies, membranes were rinsed at RT in TBST (3 ×

(3) Reducing the mitochondrial oxidative burden alleviates lipid-induced muscle insulin resistance in humansScience advancesNovember 1, 2024Fiorenza M, Onslev J, Henríquez-Olguín C, Persson KW, Hesselager SA, Jensen TE, Wojtaszewski JFP, Hostrup M, Bangsbo J.

(4) CRISPR-Cas9 screens reveal regulators of ageing in neural stem cellsNatureOctober 1, 2024Ruetz TJ, Pogson AN, Kashiwagi CM, Gagnon SD, Morton B, Sun ED, Na J, Yeo RW, Leeman DS, Morgens DW, et al.

(5) EHD2 regulates plasma membrane integrity and downstream insulin receptor signaling eventsMolecular biology of the cellNovember 1, 2023Neuhaus M, Fryklund C, Taylor H, Borreguero-Muñoz A, Kopietz F, Ardalani H, Rogova O, Stirrat L, Bremner SK, Spégel P, et al.#110 162, RRID:AB_887799) were all from Synaptic Systems (Coventry, UK). GLUT4 used for 3T3-L1 analysis (#PA1-1065 RRID:AB_2191454, Thermo Fisher Scientific Catalogue, Massachusetts, USA), insulin-regulated aminopeptidase

(6) Phosphorylation of the N-terminus of Syntaxin-16 controls interaction with mVps45 and GLUT4 trafficking in adipocytesPeerJJanuary 1, 2023Bremner SK, Berends R, Kaupisch A, Roccisana J, Sutherland C, Bryant NJ, Gould GW.described previously (Laderoute et al., 2006). Antibodies used: anti-GLUT4 (Thermo Fisher Scientific Cat# PA1-1065, RRID:AB_2191454), anti-Sx16 (Synaptic Systems Cat# 110 162, RRID:AB_887799), anti-mVps45 (Novus Cat#

(7) Overexpressing the hydroxycarboxylic acid receptor 1 in mouse brown adipose tissue restores glucose tolerance and insulin sensitivity in diet-induced obese miceAmerican journal of physiology. Endocrinology and metabolismSeptember 1, 2022Min HY, Hwang J, Choi Y, Jo YH.

(8) Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscleEndocrinology, diabetes & metabolismSeptember 1, 2022Livingstone R, Bryant NJ, Boyle JG, Petrie JR, Gould GW.proprietary software. The antibodies used in this study were anti‐GLUT4 (Thermo Fisher Scientific Cat# PA1‐1065, RRID:AB_2191454), anti‐Sx16 (Synaptic Systems Cat# 110162, RRID:AB_887799), anti‐Sx4 (Synaptic Systems

(9) EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytesBioscience reportsJuly 29, 2022Koester AM, Geiser A, Laidlaw KME, Morris S, Cutiongco MFA, Stirrat L, Gadegaard N, Boles E, Black HL, Bryant NJ, et al.#235003 and 110042, RRID:AB_887853, respectively). Anti-GAPDH [#AM4300. RRID:AB_2536381], anti-GLUT4 [# PA1-1065, RRID:AB_2191454], anti-EFR3A [#PA5-54694, RRID_AB:2640925] and anti-PI4K [#PA5-28570, RRID:AB-2546046]

(10) Chronic Hyperglycaemia Inhibits Tricarboxylic Acid Cycle in Rat Cardiomyoblasts Overexpressing Glucose Transporter Type 4International journal of molecular sciencesJune 29, 2022Stratmann B, Eggers B, Mattern Y, Silva de Carvalho T, Marcus K, Tschoepe D.anti-GLO1α (1:1000, cat. No. MA1-13029; ThermoFisher, Waltham, MA, USA); anti-GLUT4 (1:2000, cat. No. PA1-1065, ThermoFisher, Waltham, MA, USA); anti-Nrf2 total (detected as a double band, 1:1000, cat. No. PA5-68817,

(11) Muscle hypertrophic effect of inhaled beta(2) -agonist is associated with augmented insulin-stimulated whole-body glucose disposal in young menThe Journal of physiologyMay 1, 2022Jessen S, Baasch-Skytte T, Onslev J, Eibye K, Backer V, Bangsbo J, Hostrup M.Tween‐20 (TBST) before incubation overnight at 4°C in primary antibody (GLUT4; Thermo Fischer Scientific, PA1‐1065; hexokinase; Cell Signalling Technology, 2867, CS: Abcam, ab96600; COXIV: Cell Signalling Technology,

(12) Gene deletion of γ‐actin impairs insulin‐stimulated skeletal muscle glucose uptake in growing mice but not in mature adult micePhysiological ReportsFebruary 27, 2022Jonas R. Knudsen, Agnete B. Madsen, Zhencheng Li, Nicoline R. Andersen, Peter Schjerling, Thomas E. Jensen#9271), Akt (CST, #9272), p‐ULK1 Ser757 (CST, #6888), Rac1 (BD Biosciences, 61065), GLUT4 (Thermo Fisher, PA1‐1065). On the next day, membranes were incubated with the corresponding horseradish peroxidase‐conjugated

(13) Mass-spectrometry-based proteomics reveals mitochondrial supercomplexome plasticityCell reportsMay 25, 2021Gonzalez-Franquesa A, Stocks B, Chubanava S, Hattel HB, Moreno-Justicia R, Peijs L, Treebak JT, Zierath JR, Deshmukh AS.

(14) Effect of exercise training on skeletal muscle protein expression in relation to insulin sensitivity: Per-protocol analysis of a randomized controlled trial (GO-ACTIWE)Physiological reportsMay 1, 2021Bruhn L, Kjøbsted R, Quist JS, Gram AS, Rosenkilde M, Faerch K, Wojtaszewski JFP, Stallknecht B, Blond MB.#3063) and HKII (catalog #2867) were purchased from Cell Signaling Technology. Anti‐ GLUT4 (catalog #PA1‐1065; Thermo Fisher Scientific); Anti‐IR (kindly provided by Professor Ken Siddle, Cambridge University,

(15) Proteomic and Structural Manifestations of Cardiomyopathy in Rat Models of Obesity and Weight LossFrontiers in endocrinologyJanuary 1, 2021Liśkiewicz AD, Marczak Ł, Bogus K, Liśkiewicz D, Przybyła M, Lewin-Kowalik J.(Cell Signaling, #2535), anti-phospho mTOR (Thr2446 and Ser2448) (#15-105, MERK Millipore), anti-GLUT-4 (#PA1-1065, Invitrogen), anti-GLUT-1 (#ab652, Abcam), and anti-ACSL-1 (#PA5-17136, Invitrogen). After washing,

(16) Insulin-stimulated glucose uptake partly relies on p21-activated kinase (PAK)2, but not PAK1, in mouse skeletal muscleThe Journal of physiologyDecember 1, 2020Møller LLV, Jaurji M, Kjøbsted R, Joseph GA, Madsen AB, Knudsen JR, Lundsgaard AM, Andersen NR, Schjerling P, Jensen TE, et al.,Cell Signaling Technology; 9275,Rabbit; Polyclonal antibody,1:1000,2% milk GLUT4,AB_2191454,Thermo Fisher Scientific; PA1–1065,Rabbit; Polyclonal antibody,1:1000,2% milk PAK1,AB_330222,Cell Signaling Technology; 2602,Rabbit; Polyclonal

(17) Pharmacological targeting of α3β4 nicotinic receptors improves peripheral insulin sensitivity in mice with diet-induced obesityDiabetologiaJune 1, 2020Jall S, De Angelis M, Lundsgaard AM, Fritzen AM, Nicolaisen TS, Klein AB, Novikoff A, Sachs S, Richter EA, Kiens B, et al.[TBC1D] 1 Thr596 #6927 [1:1000], RRID AB_10828720); Thermo Fisher Scientific (Waltham, MA, USA) (GLUT4 #PA1-1065 [1:1000], RRID AB_2191454); Millipore (Burlington, MA, USA) (TBC1D4 #07-741 [1:1000], RRID AB_492639).

(18) Elevated MMP2 abundance and activity in mdx mice are alleviated by prenatal taurine supplementationAmerican journal of physiology. Cell physiologyJune 1, 2020Ren X, Xu H, Barker RG, Lamb GD, Murphy RM.

(19) The p21-activated kinase 2 (PAK2), but not PAK1, regulates contraction-stimulated skeletal muscle glucose transportPhysiological reportsJune 1, 2020Møller LLV, Nielsen IL, Knudsen JR, Andersen NR, Jensen TE, Sylow L, Richter EA.2,531","Rabbit, Polyclonal antibody","1:1,000",2% milk GLUT4,AB_2191454,Thermo Fisher Scientific; PA1−1065,Rabbit; Polyclonal antibody,"1:1,000",2% milk HKII,AB_2295219,Santa Cruz Biotechnology; Sc−130358,Mouse;

(20) Housing temperature influences exercise training adaptations in miceNature communicationsMarch 25, 2020Raun SH, Henriquez-Olguín C, Karavaeva I, Ali M, Møller LLV, Kot W, Castro-Mejía JL, Nielsen DS, Gerhart-Hines Z, Richter EA, et al.,Cell Signaling Technology,"1:1000, 2% skim milk",2867 GLUT4,Thermo Fisher Scientific,"1:1000, 2% skim milk",PA1-1065 TBC1D4,Abcam,"1:1000, 2% skim milk",Ab189890 OXPHOS,Abcam,"1:500, 2% skim milk",Ab110413 UCP1,Abcam,"1:1000,

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GLUT4 Antibody

GLUT4 Antibody from Thermo Fisher Scientific

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GLUT4 Antibody from Thermo Fisher Scientific

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