STAG2 (D25A4) XP ® Rabbit mAb 5882S from Cell Signaling Technology

Product Specs
ItemSTAG2 (D25A4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5882S
Quantity100 µl
ApplicationsW, IP, IF-IC, ChIP
ReactivityH, M, R, Mk
TargetSTAG2
HostRabbit
NCBI Gene AliasesHPE13, MKMS, NEDXCF, SA-2, SA2, SCC3B, bA517O1.1
ClonalityMonoclonal
NCBI Full Gene NameSTAG2 cohesin complex component
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG
CloneD25A4
Molecular Weight141

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressivenessProc Natl Acad Sci U S AApril 14, 2026Sanjana Eyunni, Shih-Chun Chu, Mary L. Guan, Michaela Louw, Eleanor Young, Sandra E. Carson, Jianhui Gong, Marcin Cieslik, Arul M. Chinnaiyan, Abhijit Paroliaauthenticate their identity. Antibodies. For immunoblotting, the following antibodies were used: STAG2(CST:5882S); EWS-Fli1 (Abcam:ab15289);H3 (Cell Signaling Technologies: 3638S); GAPDH (Cell Signaling Technologies:

(2) Sororin locks the DNA-exit gate of cohesin to preserve sister-chromatid cohesionNat CommunMarch 10, 2026Qinfu Chen, Xueying Yuan, Miao Shi, Xinyu Zhou, Shukai Zhu, Weiguo Lu, Haiyan Yan, Fangwei WangBethyl Laboratories), Pds5B (A300-537A and A300-538A, Bethyl Laboratories), SA1 (HPA035015, Sigma), SA2 (#5882, CST), Wapl (A300-268A, Bethyl Laboratories), Esco1 (29821-1-AP, Proteintech), Sororin (ab192237, Abcam),

(3) Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activationNature GeneticsFebruary 16, 2026Tessa M. Popay, Ami Pant, Femke Munting, Melodi Tastemel, Morgan E. Black, Nicholas Haghani, Jesse R. Dixon1:1000), anti-STAG1 C-terminus (Invitrogen #MA5–27807, 1:1000), anti-STAG2 N-terminus (Cell Signaling #5882, 1:1000), anti-STAG2 C-terminus (Santa Cruz #sc-81852, 1:500), and anti-CTCF (Active Motif #61932, 1:1000).

(4) Tumor suppressive activities of the cohesin SA1/STAG2 and effects of PARP impairment during Drosophila brain developmentDisease Models & MechanismsJanuary 8, 2026Simona Totaro, Antonella Lettieri, Silvia Castiglioni, Francesco Lavezzari, Cristina Gervasini, Valentina Massa, Thomas Vaccaridiluted in milk or 1× TBS-T at 4°C overnight (rabbit anti-STAG2, 1:1000, Cell Signaling Technology, 5882; rabbit anti-γH2AX, 1:1000 Cell Signaling Technology, BK9718S; rabbit anti-GAPDH, 1:1000, Cell Signaling Technology

(5) TP63 mediates the generation of tumour-specific chromatin loops that underlie MYC activation in radiation-induced tumorigenesisNature CommunicationsSeptember 30, 2025Qiaowei Liu, Shuai Jiang, Xiang Xu, Kang Xu, Yawen Luo, Zongyuan Yu, Meida Xiang, Zhouyang Xu, Lijie Wang, Sujie Zhang, et al.in this study for CUT&Tag were specific for NP63 (#67825S, 1:50, Cell Signaling Technology), STAG2 (#5882S, 1:50, Cell Signaling Technology), CTCF (#3418S, 1:50, Cell Signaling Technology), Goat Anti-Rabbit

(6) STAG2 expression imparts distinct therapeutic vulnerabilities in muscle-invasive bladder cancer cellsOncogenesisMarch 1, 2025Sarah R. Athans, Henry Withers, Aimee Stablewski, Katerina Gurova, Joyce Ohm, Anna WoloszynskaPVDF membranes. Blots were incubated with STAG2 antibody (1:500, Cell Signaling Technology Cat no. 5882) and GAPDH antibody (1:10,000, Abcam Cat no. ab9485) overnight at 4 °C, washed three times with TBST,

(7) Identification of nonsense-mediated decay inhibitors that alter the tumor immune landscapeeLifeFebruary 17, 2025Cook AL, Sur S, Dobbyn L, Watson E, Cohen JD, Ptak B, Lee BS, Paul S, Hsiue E, Popoli M, et al.monoclonal,Abcam,"ab75755,RRID:AB_1309947",Used for western blot at 1:1000 Antibody,Rabbit anti STAG2 monoclonal,Cell Signaling Technology,"5882S,RRID:AB_10834529",Used for western blot at 1:1000 Antibody,Goat anti UPF1 polyclonal,Abcam,"ab10510,RRID:AB_297251",Used

(8) One day of environment-induced heat stress damages the murine myocardiumAmerican journal of physiology. Heart and circulatory physiologyOctober 1, 2024Roths M, Rudolph TE, Krishna S, Michael A, Selsby JT.Dilution,Secondary Dilution Adenosine monophosphate-activated kinase (AMPKα),"Cell Signaling Technology (CST), No. 5882","1:1,000","1:2,000" Phospho-AMPKα (Thr172),"CST, No. 2523","1:1,000","1:2,000" ATF4 (E4Q4E),"CST,

(9) A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeresThe EMBO JournalMay 7, 2024Lu Yan, Xueying Yuan, Mingjie Liu, Qinfu Chen, Miao Zhang, Junfen Xu, Ling-Hui Zeng, Long Zhang, Jun Huang, Weiguo Lu, et al.(A11122, Invitrogen), GAPDH (14C10, Cell Signaling Technology/CST), Scc1 (ab992, Abcam), SA2 (Rabbit mAb #5882, CST), SMC1 (A300-055A, Bethyl Laboratories), SMC3 (A300-060A, Bethyl Laboratories), Pds5B (A300-537A,

(10) STAG2 Regulates Homologous Recombination Repair and Sensitivity to ATM InhibitionAdvanced ScienceNovember 20, 2023Jie Zhou, Run‐Cong Nie, Zhang‐Ping He, Xiao‐Xia Cai, Jie‐Wei Chen, Wen‐ping Lin, Yi‐Xin Yin, Zhi‐Cheng Xiang, Tian‐Chen Zhu, Juan‐Juan Xie, et al.Chemicals The antibodies used in this study were as follows: STAG2 (Cell Signaling Technology (CST) 5882S, IF, 1:200; IB, 1:1000; IHC, 1:100), BRCA1 (CST 9010S, IB, 1:1000; Santa Cruz 2996T, IF, 1:50), BARD1

(11) STAG2 expression is associated with adverse survival outcomes and regulates cell phenotype in muscle-invasive bladder cancerCancer Res CommunOctober 1, 2022Athans S, Krishnan N, Ramakrishnan S, Cortes Gomez E, Lage-Vickers S, Rak M, Kazmierczak Z, Ohm J, Attwood K, Wang J, et al.placed slides, deparaffinized, and incubated with STAG2 antibody (Cell Signaling Technology, catalog no. 5882, RRID:AB_10834529), Ki67 antibody (Abcam, catalog no. ab15580), or Cleaved Caspase 3 (Cell Signaling Technology

(12) Stromal Antigen 2 Deficiency Induces Interferon Responses and Restricts Porcine Deltacoronavirus InfectionVirusesAugust 15, 2022Yang Wu, Hongling Zhang, Jianfei Chen, Zhaorong Shi, Mingwei Li, Ying Zhao, Hongyan Shi, Da Shi, Longjun Guo, Li Fengsequencing. The listed antibodies were used in this study including the STAG2 rabbit monoclonal antibody (mAb) (5882) and a phospho-STAT1 (Tyr701) (D4A7) rabbit mAb (7649) were purchased from Cell Signaling Technology,

(13) Systematic discovery of mutation-directed neo-protein-protein interactions in cancerCellMay 26, 2022Mo X, Niu Q, Ivanov AA, Tsang YH, Tang C, Shu C, Li Q, Qian K, Wahafu A, Doyle SP, et al.antibody,Cell Signaling,Cat# 2873; RRID:AB_2062659 Rabbit polyclonal anti-STAG2 antibody,Cell Signaling,Cat# 5882; RRID:AB_10834529 Rabbit polyclonal anti-p38-delta (MAPK13) antibody,Cell Signaling,Cat# 2308; RRID:AB_10694398 Rabbit

(14) STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcomaCancer CellJune 14, 2021Biniam Adane et al.(Western blot),Santa Cruz,Cat#sc-81852; RRID: AB_2199948 Rabbit anti-STAG2 (ChIP-seq),Cell Signaling,Cat#5882; RRID:AB_10834529 Rabbit anti-STAG1(Western blot),Bethyl,Cat# A302-579A;RRID:AB_2034857 Rabbit anti-STAG1(ChIP-seq),Bethyl,Cat#

(15) Regulation of sister chromatid cohesion by nuclear PD-L1Cell researchJuly 1, 2020Yu J, Qin B, Moyer AM, Nowsheen S, Tu X, Dong H, Boughey JC, Goetz MP, Weinshilboum R, Lou Z, et al.Origene. Anti-SMC1 antibody (4802S), anti-SMC3 antibody (5696S), anti-SCC1 antibody (4321S), anti-STAG2 antibody (5882S), anti-WAPL antibody (77428S) and anti-PD-L1 antibody (13684S, for Western blot) were purchased from

(16) Mutations in the sonic hedgehog pathway cause macrocephaly‐associated conditions due to crosstalk to the PI3K/AKT/mTOR pathwayAmerican Journal of Medical Genetics Part ADecember 1, 2019Steven D. Klein, Dzung C. Nguyen, Viraj Bhakta, Derek Wong, Vivian Y. Chang, Tom B. Davidson, Julian A. Martinez‐AgostoTween (TBST) for 30 min, then incubated in primary antibodies for 72 hr against STAG2 (Cell Signaling #5882, Dilution 1/500) and beta-actin (Cell Signaling #4970, Dilution 1/5000). Blots were then rinsed with

(17) TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanismsCellular and molecular life sciences : CMLSJuly 1, 2018Rosengren T, Larsen LJ, Pedersen LB, Christensen ST, Møller LB.

(18) STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infectionNature communicationsApril 16, 2018Ding S, Diep J, Feng N, Ren L, Li B, Ooi YS, Wang X, Brulois KF, Yasukawa LL, Li X, et al.p-IRF3 Ser396 (CST, D6O1M, #29047, 1:200), NSP2 (clone 191, 1:200)-Alexa Fluor 488, STAG2 (CST, D25A4, #5882), VP6 (clone 1E11, 1:200)-Alexa Fluor 488, MitoTracker Red CMXRos (Thermo Fisher, M7512), and Phalloidin-Alexa

(19) Synthetic lethality between the cohesin subunits STAG1 and STAG2 in diverse cancer contextseLifeJuly 10, 2017van der Lelij P, Lieb S, Jude J, Wutz G, Santos CP, Falkenberg K, Schlattl A, Ban J, Schwentner R, Hoffmann T, et al.Montgomery, TX, US; A300-158A and A300-159A), N-term. mAb rabbit anti-STAG2 (Cell Signaling, Danvers, MA, US; 5882), full length pAb rabbit anti-STAG2 (Cell Signaling, Danvers, MA, US; 4239), mouse anti-GAPDH (Abcam,

(20) The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ9Vδ2 T cell activation by dendritic cellsNature communicationsJune 5, 2017Castella B, Kopecka J, Sciancalepore P, Mandili G, Foglietta M, Mitro N, Caruso D, Novelli F, Riganti C, Massaia M.

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STAG2 (D25A4) XP ® Rabbit mAb

STAG2 (D25A4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (23)

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STAG2 (D25A4) XP ® Rabbit mAb from Cell Signaling Technology

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