Streptavidin-HRP 3999S from Cell Signaling Technology

Product Specs
ItemStreptavidin-HRP
CompanyCell Signaling Technology
Price $173.00 Inquire
Catalog Number3999S
Size1 ml
ApplicationsW
Conjugate/TagHRP
Research AreaRelated Products
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Modified protein selection strategy based on Escherichia coli’s Hitchhiker transport and validation through selection of nanobodies targeting bovine interferon gammaScientific ReportsMarch 13, 2026Suwitchaya Sirimanakul, Joseph D. Hurley, Apisitt Thaiprayoon, Kwanpet Intasurat, Warisara Kasemsukwimol, Nonth Submunkongtawee, Lueacha Tabtimmai, Kittitat Jaengwang, Kiattawee Choowongkomon, Pinpunya Riangrungroj, et al.Biotinylated anti-bIFN-γ (MCA1783B, Bio-Rad, USA) as the primary antibody and 1:1,000 Streptavidin: HRP (3999 S, Cell Signaling Technology, USA) as the secondary antibody, or 1:500 Mouse mAb to β-lactamase (ab12251,

(2) Profiling the pancreatic cancer secretome with metabolic glycoengineeringJournal of Biological ChemistryMarch 1, 2026Kris Dammen-Brower, Stanley Zhu, Christian Agatemor, Safiya Aafreen, Vrinda Dharharma, Christopher T. Saeui, Hui Li, Jian Song, Matthew J. Buettner, Keith R. Kwagala, et al.Tween-20 (TBST) for 1 h, and probed with streptavidin–horseradish peroxidase (HRP) (Cell Signaling, 3999S) in 5% (w/v) bovine serum albumin in TBST used at a ratio of 1:10,000 or 1:150,000 for proteins extracted

(3) Identifying regulators of associative learning using a protein-labelling approach in Caenorhabditis eleganseLifeJanuary 28, 2026Aelon Rahmani, Anna McMillen, Ericka Allen, Radwan Ansaar, Renee Green, Michaela E Johnson, Anne Poljak, Yee Lian Chew(1:20,000; Thermo Fisher Scientific, #G-21040), and streptavidin-HRP (1:5000; Cell Signalling Technology, #3999; Jeong et al., 2018; Stefanoska et al., 2022). Probed proteins were visualised by chemiluminescence

(4) Identifying regulators of associative learning using a protein-labelling approach in Caenorhabditis elegansElifeJanuary 28, 2026Aelon Rahmani, Anna McMillen, Ericka Allen, Radwan Ansaar, Renee Green, Michaela E Johnson, Anne Poljak, Yee Lian Chew(1:20,000; Thermo Fisher Scientific, #G-21040), and streptavidin-HRP (1:5000; Cell Signalling Technology, #3999; Jeong et al., 2018; Stefanoska et al., 2022). Probed proteins were visualised by chemiluminescence

(5) IMM2510, a novel anti-PD-L1/VEGF bispecific antibody for cancer immunotherapyAntibody TherapeuticsJanuary 15, 2026Dianze Chen, Zhuli Wu, Xiwen Zhao, Rupal S Bhatt, Yanan Yang, Wenqi Zhu, Huang Tang, Kaili Wang, Chunli Guo, Dandan Liu, et al.and washing, biotinylated human VEGF-hFc was added. HRP-conjugated streptavidin (Cell Signaling, Cat#3999S) was added and incubated. Detection was performed as described in section 2.4. Blocking assay by ELISA To

(6) Enhancing anti-tumor immunity through co-blocking PD-L1 and TIGIT by facilitating tumor-directed responses and additional VEGF inhibitionFrontiers in ImmunologyJanuary 14, 2026Xi Zhu, Xiaopei Cui, Haijia Yu, Jingen Xu, Xiaofang Chen, Xiaochen Ren, Xiaoyue Wei, Shi Chen, Yangtin Wang, Liyang Fei, et al.incubated for 2 hours, followed by three washes. Detection was performed with streptavidin-HRP (CST, Cat#: 3999S), and plates were washed six times. TMB substrate (BioLegend, Cat#: 421101) was added, followed by stop

(7) Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional programNature CommunicationsJanuary 8, 2026Nana Zhang, Ran Zhao, Xiaomin Zhong, Qian Dong, Yajie Liu, Kairan Yu, Lirui Han, Fanxu Meng, Jiaxuan Wu, Qiushi Chen, et al.IgG-HRP (CST, #7074, 1:20,000), anti-mouse IgM-HRP (Abcam, #ab97230, 1:20,000), Streptavidin-HRP (CST, #3999, 1:50,000), and the signals were detected by the ECL Plus kit (GE Health-care). All immunoblot experiments

(8) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.milk, incubated with streptavidin labeled with horseradish peroxidase (HRP; Cell Signaling Technology, 3999), and visualized using a Protein Imaging System (Tanon, 5200Multi). The oligonucleotide sequences used

(9) Photo-proximity labeling in live primary neurons identifies an AMPA-receptor signal transducer in homeostatic synaptic plasticityCell Chemical BiologyDecember 18, 2025Avik Basu, Yuan Tian, Sergei Kotelnikov, Gabriel M. Cohn, Sadhna Phanse, Dima Kozakov, Heng-Ye Man, Andrew Emilithe membrane was directly probed with HRP conjugated streptavidin (Cell Signaling Technology cat. #3999S) for 3 hours at room temperature and imaged using chemiluminescence detection. Proximity Ligation Assay

(10) Integrating endogenous TurboID and data-independent acquisition mass spectrometry for in vivo proximity labelingThe EMBO JournalDecember 11, 2025David S Fay, Boopathi Balasubramaniam, Sean M Harrington, Philip T Edeenorder) as needed. Antibodies used in this study were Streptavidin-HRP (Cell Signaling Technology, Cat# 3999S), β-Actin (13E5)-HRP (Cell Signaling Technology, Cat# 5125S), and THE DYKDDDDK [FLAG-TAG] Antibody-HRP

(11) Mechanistic Characterization of a Small Molecule as a Direct NLRP3 Inhibitor via Binding to the NACHT DomainJournal of Medicinal ChemistryNovember 27, 2025Yiming Xu, Hallie Blevins, Savannah Biby, Jannatun N. Namme, Kun Zhang, Renfeng Li, Shijun Zhangcaspase-1 antibody (E2G2I) (1:1,000), GAPDH antibody (14C10) (1:1,000), and streptavidin-HRP antibody (3999) (1:2,000) were obtained from Cell Signaling. Secondary HRP-conjugated antibodies used were antirabbit

(12) COCA-seq: genome-wide mapping of O-GlcNAc-associated open chromatin: COCA-seq maps O-GlcNAcylated open chromatinActa Biochimica et Biophysica SinicaNovember 24, 2025Chang Ge, Ran Zhao, Hongyu Jiang, Qingbin Chen, Zhentao Yu, Hankai Yang, Xuan Jiang, Qile Ma, Lirui Han, Kairan Yu, et al.Biotinylated proteins were detected by incubating with streptavidin-conjugated horseradish peroxidase (#3999; Cell Signaling Technology, Beverly, USA) diluted 1:20,000 in PBST for 45 min at room temperature followed

(13) Development of Single‐Molecule Enzyme Activity Assay for Serine Hydrolases Using Activity‐Based Protein Labeling ProbesSmall MethodsNovember 1, 2025Seiya Ishii, Mayano Minoda, Tadahaya Mizuno, Takumi Iwasaka, Hiroyuki Kusuhara, Kazufumi Honda, Yasuteru Urano, Toru Komatsucontaining Tween‐20 (0.05%; TBS‐T), and incubated with Streptavidin‐HRP (Cell Signaling Technology #3999, 1/3000 in TBS‐T) for 1 h. The membrane was washed three times with TBS‐T. The HRP‐linked antibody was

(14) TurboID‐Mediated Profiling of Glioblastoma‐Derived Extracellular Vesicle Cargo ProteinsJournal of Extracellular VesiclesSeptember 23, 2025Marissa N. Russo, Emily S. Norton, Maria José Ulloa‐Navas, Natanael Zarco, Weiwei Wang, TuKiet T Lam, Alfredo Quiñones‐Hinojosa, Veronique V. Belzil, Hugo Guerrero‐Cázares#SC‐12740), HSP90B1 (1:1000, Cell Signaling #7411), and Streptavidin HRP (1:10,000, Cell Signaling #3999) all diluted in 5% BSA. V5 (1:1000, Thermo Scientific #R960‐25) and anti‐albumin (1:1000, Fortis life

(15) Opto-CD28-REACT: optogenetic co-stimulatory receptor activation on non-engineered human T cellsFrontiers in ImmunologySeptember 19, 2025Anna K. Ehret, Sara Hartmann, Pavel Salavei, Virginia Andreani, Nicole Gensch, Laura Gámez-Díaz, Wolfgang W. Schamelblocking buffer or HRPO-conjugated anti-streptavidin antibody (Cell Signaling Technologies, Cat. No. 3999S) diluted 1:2,000 in wash buffer supplemented with 3% BSA and 0.02% NaN3. Proteins were detected via

(16) RAD23B acquires a copper metalloadaptor function in amphibian-to-reptile evolution to increase metabolism and regulate genomic integrityMolecular CellSeptember 18, 2025Tong Xiao, Dan He, Danqian Liu, Shang Jia, Qingyi Chen, Daniel Silverman, Neilabjo Maitra, Alan Y. Huang, Aidan Pezacki, Trisha T. Nguyen, et al.conjugate,Jackson ImmunoResearch,Cat# 016-030-084; RRID:AB_2337238 Streptavidin-HRP,Cell Signaling Technology,Cat# 3999; RRID:AB_10830897 V5-tag,Abcam,Cat# ab206566; RRID:AB_2819156 CTR1,Proteintech,Cat# 67221-1-lg; RRID:AB_2882512 CTR1,Proteintech,Cat#27499-1-AP;

(17) HIF2-driven PTHrP Causes Cachexia and Hypercalcemia in Kidney Cancer: Treatment with HIF2 InhibitorsbioRxivSeptember 16, 2025Muhannad Abu-Remaileh, Laura A. Stransky, Nikita Bhalerao, Nitin H. Shirole, Qinqin Jiang, Eddy Saad, Marc Machaalani, Sean M. Vigeant, Hilina Woldemichael, Charles Xu, et al.anti-b-Actin (Cell Signaling Technology; 3700S), anti-streptavidin-HRP (Cell Signaling Technology; 3999S), Rabbit anti-IGFBP3 (Cell Signaling Technology; 25864S), and Rabbit anti-MMP2 (D2O4T) (Cell Signaling Technology

(18) Mesoscale regulation of microtubule-organizing centers by the E3 ligase TRIM37Nature Structural & Molecular BiologySeptember 1, 2025Zhong Y. Yeow, Sonia Sarju, Fang-Chi Chang, Lance Y. Xu, Mark van Breugel, Andrew J. Holland#7077; 1:1000), anti-rabbit (goat; Cell Signaling, #7074, 1:1000), and streptavidin (Cell Signaling, #3999; 1:1500), with SuperSignal West Pico PLUS or Femto Maximum chemiluminescence substrate (Thermo Fisher

(19) Nascent liver proteome reveals enzymes and transcription regulators under physiological and alcohol exposure conditionsNat CommunAugust 26, 2025Jiayu Gu, Lihui Lao, Linzhen Hu, Jia Zang, Chao Liu, Ruixi Wan, Ling Tang, Ying Yuan, Yulin Chen, Shixian LinCell Signaling Technology), anti-Ubiquitin (10201-2-AP, Proteintech), and anti-Streptavidin-HRP antibody (3999, Cell Signaling Technology) were used at a dilution of 1:1000. Secondary antibodies: IgG mouse (SA00001-1,

(20) Assembly and Mutagenesis of Human Coronavirus OC43 Genomes in Yeast via Transformation-Associated RecombinationBio ProtocAugust 20, 2025Brett A. Duguay, Craig McCormickP9416) 52. ssRNA ladder (NEB, catalog number: N0362S) 53. Streptavidin-HRP (Cell Signaling, catalog number: 3999) 54. Odyssey Blocking Buffer (LI-COR, catalog number: 927-50000) 55. Clarity Max Western ECL Substrate

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Streptavidin-HRP

Streptavidin-HRP from Cell Signaling Technology

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Cell Signaling Technology

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Streptavidin-HRP from Cell Signaling Technology

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