Propidium Iodide (PI)/RNase Staining Solution from Cell Signaling Technology

Product Specs
ItemPropidium Iodide (PI)/RNase Staining Solution
CompanyCell Signaling Technology
Price $156.00 Inquire
Catalog Number4087S
Quantity100 ml
Research AreaRelated Products
ApplicationsIF-P, IF-F, IF-IC, FC-FP
ReactivityAll

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Modeling of Capicua Family Fusion Oncoprotein-Driven Cancers Reveals Gene-Specific FunctionalityMol Cancer ResMay 4, 2026Cuyler Luck, Yongfeng Luo, Elena Vasileva, Kyle A. Jacobs, Julia Riad, Christopher D. Macaraig, Rovingaile Kriska M. Ponce, James F. Amatruda, Ross A. OkimotoSamples were then pelleted and resuspended in propidium iodide staining buffer (Cell Signaling Technology 4087) at a volume of 500 uL per 1 million cells. An untransfected, unstained sample was included as a negative

(2) EMT activates ER-to-Golgi trafficking through upregulation of REEP2 to promote lung cancer progressionbioRxivMarch 27, 2026Kevin Fulp, Oluwafunminiyi Obaleye, Shike Wang, Xin Liu, Jiang Yu, Jonathan M. Kurie, Jun Xu, William K. Russell, Xiaochao Tan, Guan-Yu Xiao70% ethanol at 4°C overnight, and were incubated with Propidium Iodide (PI)/RNase Staining Solution (#4087S, Cell Signaling Technologies) for 30 min. After incubation, cell cycle was analyzed using a flow cytometer

(3) Inhibition of USP7 destabilizes the noncanonical PRC1.1 complex and induces neuroblastoma differentiationMolecular Cancer ResearchMarch 19, 2026Emily A. Cmarik, Abhishek Wahi, Sayali S. Chandekar, Solomon K. Otchere, Jennifer E. Sturgis, Silvi Salhotra, Allen T. Basanthakumar, Nehemiah X. Boyd, Giulia DiGiovanni, He Zhu, et al.saponin wash supplemented with 5% propidium iodide/RNase staining solution (Cell Signaling Technology, 4087). The samples were transferred into FACS tubes and then analyzed using a BD LSRFortessa cell analyzer. ChIP sequencing

(4) Inhibition of USP7 destabilizes the noncanonical PRC1.1 complex and induces neuroblastoma differentiationMol Cancer ResMarch 19, 2026Emily A. Cmarik, Abhishek Wahi, Sayali S. Chandekar, Solomon K. Otchere, Jennifer E. Sturgis, Silvi Salhotra, Allen T. Basanthakumar, Nehemiah X. Boyd, Giulia DiGiovanni, He Zhu, et al.saponin wash supplemented with 5% propidium iodide/RNase staining solution (Cell Signaling Technology, 4087). The samples were transferred into FACS tubes and then analyzed using a BD LSRFortessa cell analyzer. ChIP sequencing

(5) Oncostatin-M ligand-based CAR-T therapy displays robust anti-tumor activity against osteosarcomaBMC MedMarch 17, 2026Daniel Feinberg, Vinayak Uppin, Saada Eid, Claire Fritz, Masahiro Hitomi, Akshaya Radhakrishnan, Kayla Klatt, Sung Hee Choi, Jay T Myers, Aarshvi Bhatt, et al.spun at 400 g × 5 min, washed with PBS, spun again, and stained for 15 min with propidium iodide (PI) (4087, Cell Signaling) covered from light at room temperature (RT). Cells were then analyzed via flow cytometry

(6) WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopmentCell Death DiscoveryMarch 6, 2026Ki Hurn So, Seungbok Lee, Jiayi Wong, Hyunsik Lee, Eun-Jin Yun, Se Song Jang, Hee-Jung Choi, Jong-Hee Chae, Seung Tae Baekdetermined by the cell viability using Propidium Iodide/RNase staining solution (Cell Signaling Technology, #4087). Non-toxic concentrations of treatments were added together with NucView® caspase-3 substrates upon

(7) MicroRNA-15a-5p suppresses triple-negative breast cancer cell proliferation and invasion by modulating T-cell immunoreceptor associated with immunoglobulin and ITIM domain expressionCytojournalJanuary 31, 2026Shaopeng Feng, Yunjie Song, Jintao Cui, Zhihao Wu, Limin Weimaintained at 4°C overnight. The cells were rinsed twice with PBS, treated with 100 μL of RNase A (#4087; Cell Signaling Technology, USA) at 37°C for 30 min, and incubated with 400 μL of propidium iodide (PI)

(8) MicroRNA-15a-5p suppresses triple-negative breast cancer cell proliferation and invasion by modulating T-cell immunoreceptor associated with immunoglobulin and ITIM domain expressionCytojournalJanuary 31, 2026Shaopeng Feng, Yunjie Song, Jintao Cui, Zhihao Wu, Limin Weimaintained at 4°C overnight. The cells were rinsed twice with PBS, treated with 100 μL of RNase A (#4087; Cell Signaling Technology, USA) at 37°C for 30 min, and incubated with 400 μL of propidium iodide (PI)

(9) Modeling of Capicua Family Fusion Oncoprotein-Driven Cancers Reveals Gene-Specific FunctionalityMolecular Cancer ResearchJanuary 23, 2026Cuyler Luck, Yongfeng Luo, Elena Vasileva, Kyle A. Jacobs, Julia Riad, Christopher D. Macaraig, Rovingaile Kriska M. Ponce, James F. Amatruda, Ross A. OkimotoSamples were then pelleted and resuspended in propidium iodide staining buffer (Cell Signaling Technology 4087) at a volume of 500 uL per 1 million cells. An untransfected, unstained sample was included as a negative

(10) PTEN-loss confers dependence on the guanylate synthesis enzyme IMPDH in T-cell acute lymphoblastic leukemiaBIORXIVDecember 24, 2025Rayees A. Padder, Thu Le Le, Emily Hyde, Maria E. D. Rubio, Eric Chiles, Namratha Sheshadri, Kelly Mulraney, Wei-Xing Zong, Xiaoyang Su, Daniel Herranz, et al.followed by washing with 1X PBS. Cells were then stained with propidium iodide-RNase solution (CST #4087) for 1 hour at room temperature. PI staining intensity was measured on a Gallios Flow Cytometer (Beckman

(11) A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesisScienceDecember 11, 2025Tuan-Anh Nguyen, Jung-Ming G. Lin, Anne-Sophie M. C. Marques, Maximilian Fottner, Ludwig G. Bauer, Andreas Reicher, Diana Daum, Lorenzo Scrofani, Yusi Liu, Carol Cheng, et al.were resuspended in PBS for 5 min and then stained with propidium iodide (Cell Signaling Technology, #4087S) according to manufacturer’s protocol. DNA content was measured with Sony SH800S cell sorter and analyzed

(12) B-cell activating factor plays a critical role in CAR-T cell-associated cytokine release syndromebioRxivNovember 27, 2025Claire Fritz, Leland Metheny, David Wald, Paolo Caimi, Reshmi ParameswaranAfter 16h of co-culture, the cells were stained with propidium iodide (PI, Cell Signaling Technology, 4087S), and cancer cell death was measured by the APC (eFlour670) + PE (PI)+ cell population. The supernatant

(13) Efficacy of Wee1 G2 Checkpoint Kinase and Mouse Double Minute 2 Homolog Inhibitors in Gastrointestinal Stromal Tumors Determined by p53 StatusOncology ResearchOctober 22, 2025Chiao-Ping Chen, Yan-Jei Tang, You-Yan Cai, Yi-Ru Pan, Chun-Nan Yeh, Wen-Kuan Huang, Chih-Hong Lo, Yu-Tien Hsiao, Hsuan-Jen Shih, Chiao-En Wuethanol, and incubated with Propidium Iodide (PI)/RNase Staining Solution (Cell Signaling Technology, #4087) for 20 min in the dark at room temperature. The samples were then analyzed for DNA content using a

(14) Efficacy of Wee1 G2 Checkpoint Kinase and Mouse Double Minute 2 Homolog Inhibitors in Gastrointestinal Stromal Tumors Determined by p53 StatusOncology ResearchOctober 22, 2025Chiao-Ping Chen, Yan-Jei Tang, You-Yan Cai, Yi-Ru Pan, Chun-Nan Yeh, Wen-Kuan Huang, Chih-Hong Lo, Yu-Tien Hsiao, Hsuan-Jen Shih, Chiao-En Wuethanol, and incubated with Propidium Iodide (PI)/RNase Staining Solution (Cell Signaling Technology, #4087) for 20 min in the dark at room temperature. The samples were then analyzed for DNA content using a

(15) Multiplexed epigenetic memory editing using CRISPRoff sensitizes glioblastoma to chemotherapyNeuro-OncologyJuly 30, 2025Katie Lin, Christopher Zou, Akane Hubbard, Sasha Sengelmann, Laine Goudy, I-Ching Wang, Rohit Sharma, Joanna Pak, Kyla Foster, Tomoko Ozawa, et al.performed by 70% ethanol fixation followed by propidium iodide staining (Cell Signaling Technology, cat 4087S) analyzed on the Attune NxT Flow Cytometer 48 hours after treatment with the drug. Analysis of apoptosis

(16) RNA-binding protein gene NOP58 exhibits crucial prognostic and therapeutic value in Ewing sarcomaHereditasMay 14, 2025Yannan Geng, Lu Yang, Rui Shao, Tiantong Xu, Lilong Zhang16 h. Flow cytometry for cell cycle analysis For cell cycle analysis, a cell cycle detection kit (4087 S, CST, Danvers, MA, US) was used. Briefly, cells were suspended in ice-cold phosphate buffered saline

(17) Ribonuclease 1 Induces T-Cell Dysfunction and Impairs CD8(+) T-Cell Cytotoxicity to Benefit Tumor Growth through Hijacking STAT1Advanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2025Yang WH, Huang BY, Rao HY, Ye P, Chen B, Wang HC, Chung CH, Wu HH, Yen HR, Wang SC, et al.Analysis Cell cycle analysis was performed by using propidium iodide (PI)/RNase staining solution (#4087; Cell Signaling Technology) according to manufacturer's protocol. Briefly, 5 × 105 cells were collected

(18) Small extracellular vesicles enhance the survival of Sca-1+ cardiac stem cells against ROS-induced ischemic-reoxygenation injury in vitroBiol ResMarch 5, 2025Radwa A. Mehanna, Hagar Elkafrawy, Marwa M. Essawy, Samar S. Ibrahim, Ashraf K. Awaad, Nehal A. Khalil, Marwa A. Kholief, Abeer Sallam, Heba A. Hamed, Mona A. Barkat, et al.9.1.B). Cell proliferation Cell cycle assay Cell cycle assay was performed by PI/RNase reagent (cat# 4087 S, Cell Signaling, Life Technology, USA). Briefly, cells were centrifuged, washed, and resuspended in

(19) E2F1-mediated ESPL1 transcriptional activation predicts poor prognosis and promotes the proliferation of leiomyosarcomaCytojournalJanuary 8, 2025Xiaojuan Yang, Guihua Miao, Qin Wang, Qin Yu, Qinsheng Hu, Gang Tancells were fixed in ice-cold ethanol, stained with propidium iodide (PI) solution containing RNase A (#4087; Cell Signaling Technology, USA), and analyzed using a BD Accuri C6 flow cytometer. The cell cycle phases

(20) E2F1-mediated ESPL1 transcriptional activation predicts poor prognosis and promotes the proliferation of leiomyosarcomaCytojournalJanuary 8, 2025Xiaojuan Yang, Guihua Miao, Qin Wang, Qin Yu, Qinsheng Hu, Gang Tancells were fixed in ice-cold ethanol, stained with propidium iodide (PI) solution containing RNase A (#4087; Cell Signaling Technology, USA), and analyzed using a BD Accuri C6 flow cytometer. The cell cycle phases

Be the First to Write a Review!