Merlin (D1D8) Rabbit mAb 6995S from Cell Signaling Technology

Product Specs
ItemMerlin (D1D8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number6995S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R
TargetMerlin
IsotypeIgG
HostRabbit
NCBI Gene AliasesACN, BANF, SCH, merlin-1
NCBI Full Gene NameNF2, moesin-ezrin-radixin like (MERLIN) tumor suppressor
CloneD1D8
Molecular Weight70
ClonalityMonoclonal
Research AreaNeuroscience

Questions and Answers

Can this antibody be used to study the role of Merlin in cancer biology?

Yes, the Merlin (D1D8) Rabbit mAb has been utilized in various studies to investigate its role as a tumor suppressor in cancer. For instance, it has been referenced in research focusing on the mechanisms of tumor growth and progression, particularly in the context of pancreatic cancer and its interaction with signaling pathways such as the Hippo pathway ().

What applications is this antibody suitable for in experimental protocols?

The Merlin (D1D8) Rabbit mAb is suitable for Western blotting and immunoprecipitation applications. It has been effectively used in these contexts to analyze Merlin protein levels and interactions in various cell types, as demonstrated in multiple studies (, ).

Is this antibody effective for use in mouse models?

Yes, the Merlin antibody has shown reactivity with mouse tissues, making it suitable for studies involving murine models. It has been cited in research that examines the role of Merlin in mouse models of cancer, particularly in understanding tumor microenvironments and signaling pathways ().

How does the antibody perform in detecting Merlin protein levels?

The Merlin (D1D8) Rabbit mAb is designed to recognize endogenous levels of total Merlin protein, which has been validated in various studies. It has been used to assess Merlin expression in different cellular contexts, including its downregulation during differentiation processes in neuronal cells ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Strategic selection of MDM2 inhibitors enhances the efficacy of FAK inhibition in mesothelioma based on TP53 genotypePLOS OneFebruary 23, 2026Xuerao Ning, Thảo Thi Thanh Nguyễn, Takao Morinaga, Yuji Tada, Hideaki Shimada, Kenzo Hiroshima, Naoto Yamaguchi, Masatoshi Tagawa(#3285), phosphorylated FAK at Tyr 397 (#3283), phosphorylated MDM2 at Ser 166 (#3521), MERLIN (D1D8, #6995), actin (#4970) (Cell Signaling, Danvers, MA, USA), phosphorylated H2AX (phosphor-2A histone family

(2) De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesotheliomaEMBO Molecular MedicineJuly 24, 2025Duo Xu, Yanyun Gao, Shengchen Liu, Shiyuan Yin, Tong Hu, Haibin Deng, Tuo Zhang, Balazs Hegedüs, Thomas M Marti, Patrick Dorn, et al.vector Antibodies,Antibodies,Antibodies Rabbit anti-NF2,Proteintech,21686-1-AP Rabbit anti-NF2,CST,6995 Rabbit anti-CAD,Proteintech,16617-1-AP Rabbit anti-DHODH,Proteintech,14877-1-AP Rabbit anti-YAP,CST,14074S Rabbit

(3) An induced pluripotent stem cell model of Schwann cell differentiation reveals NF2- related gene regulatory networksResearchSquareJune 16, 2025Olivia Lazaro, Sihong Li, William Carter, Jake Smiley, Oluwamayowa Awosika, Sylvia Robertson, Angela Haskell, Raven Hinkel, Brooke E. Hickey, Steven P. Angus, et al.Sanger sequencing. Merlin protein expression was evaluated by western blot using Merlin antibody (Cell Signalling #6995S). Multiple heterozygous clones were obtained, and then we proceeded with three selected NF2 knockout

(4) The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cellsCell ReportsMarch 25, 2025Michael R. Weaver, Dominika Shkoruta, Marta Pellegatta, Caterina Berti, Marilena Palmisano, Scott Ferguson, Edward Hurley, Julianne French, Shreya Patel, Sophie Belin, et al.ab52641), rabbit anti-p-Mst1/2 1:250 (Proteintech 28953–1-AP), rabbit anti-NF2 1:500 (Cell Signaling 6995), rabbit anti-p-NF2 1:500 (Cell Signaling 1328), rabbit anti-Oct6/Pou3f1 1:500 (kindly shared by D.

(5) PI4P-mediated solid-like Merlin condensates orchestrate Hippo pathway regulationScienceAugust 9, 2024Pengfei Guo, Bing Li, Wei Dong, Huabin Zhou, Li Wang, Ting Su, Christopher Carl, Yonggang Zheng, Yang Hong, Hua Deng, et al.permeabilization in PBST. Fixed cells were then incubated with primary (Cell Signaling Technology Cat# 6995, RRID:AB_10828709, 1:500) and second antibodies similar to the immunostaining of fly tissues. Western blot

(6) Synergistic effect of PAK and Hippo pathway inhibitor combination in NF2-deficient SchwannomaPLOS ONEJuly 31, 2024Dorothy Benton, Hoi Yee Chow, Sofiia Karchugina, Jonathan Chernofffor PAK1 (#2606), PAK2 (#2608), Phospho-PAK1 (Ser144)/PAK2 (Ser141) (#2606), YAP1 (#4912), Merlin (#6995), GAPDH (#2118), and β-actin (#4970) were purchased from Cell Signaling Technologies. Blots were incubated

(7) Loss of tumor suppressors promotes inflammatory tumor microenvironment and enhances LAG3+T cell mediated immune suppressionNature communicationsJuly 12, 2024Zahraeifard S, Xiao Z, So JY, Ahad A, Montoya S, Park WY, Sornapudi T, Andohkow T, Read A, Kedei N, et al.(abcam, ab17963), TSC1 (ThermoFisher, PA5-20131), TGF-βR2 (R&D System, AF532), NF2 (Cell Signaling, #6995, D1D8), Pten (Cell Signaling, #9559, 138G6), Caspase 3 (Cell Signaling, #9665, 8G10), pAKT (Cell Signaling,

(8) Development of Novel Bioluminescent Biosensors Monitoring the Conformation and Activity of the Merlin Tumour SuppressorInternational Journal of Molecular SciencesJanuary 26, 2024Alexander Pipchuk, Tynan Kelly, Madeleine Carew, Christopher Nicol, Xiaolong Yangmembranes were blocked with 5% skim milk for 1 h at RT, then incubated with anti-Merlin (Cell Signalling #6995; diluted 1:1000), anti-β-actin (Sigma–Aldrich #A5441; diluted 1:10,000), or anti-Myc (Cell Signalling

(9) TIMAP, a Regulatory Subunit of Protein Phosphatase 1, Inhibits In Vitro Neuronal DifferentiationInt J Mol SciDecember 11, 2023Márton Fonódi, Zsófia Thalwieser, Csilla Csortos, Anita Boratkó(A5060)" ERM,1:1000,"Cell Signaling Technologies (Danvers, MA, USA) (#3142)" merlin,1:1000,Cell Signaling Technologies (#6995) RACK1,1:1000,"BD Transduction Laboratories (Franklin Lakes, NJ, USA) (#610177)" GFP,1:1000,Millipore

(10) PIP5Kγ Mediates PI(4,5)P2/Merlin/LATS1 Signaling Activation and Interplays with Hsc70 in Hippo-YAP Pathway RegulationInternational journal of molecular sciencesSeptember 30, 2023Le DDT, Le TPH, Lee SY.(#13202), LATS1 (#9153), phospho-LATS1 Ser909 (#9157), YAP (#4912), phospho-YAP Ser127 (#4911), Merlin (#6995), and PIP5Kγ (#3296) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against

(11) Cellular mechanisms of heterogeneity in NF2-mutant schwannomaNature communicationsMarch 21, 2023Chiasson-MacKenzie C, Vitte J, Liu CH, Wright EA, Flynn EA, Stott SL, Giovannini M, McClatchey AI.mouse mAb (1:1000; clone AC-40, #A4700, Millipore Sigma); anti-merlin rabbit mAb (1:100; clone D1D8, #6995, Cell Signaling Technology); anti-pS6 (S235/236) rabbit pAb (1:100; #2211, Cell Signaling Technology);

(12) JAM-A signals through the Hippo pathway to regulate intestinal epithelial proliferationiScienceMay 20, 2022Fan S, Smith MS, Keeney J, O'Leary MN, Nusrat A, Parkos CA.Anti-p-LATS1 S909,Cell Signaling Technology,9157; RRID: AB_2133515 Rabbit Anti-NF2,Cell Signaling Technology,6995; RRID: AB_10828709 Rabbit Anti-NF2,Cell Signaling Technology,12888; RRID: AB_2650551 Rabbit Anti-p-NF2/Merlin

(13) Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin statusScience advancesOctober 1, 2021Murphy KJ, Reed DA, Vennin C, Conway JRW, Nobis M, Yin JX, Chambers CR, Pereira BA, Lee V, Filipe EC, et al.,Cell Signaling Technology,9661,1:200,60 pTyr397-FAK,Abcam,ab39967,1:4000,30 Merlin (NF2) (D1D8),Cell Signaling Technology,6995,1:1000,60 GFP,Invitrogen,A11122,1:1000,60 Primary antibodies used for IHC, with staining performed

(14) The effects of palbociclib in combination with radiation in preclinical models of aggressive meningiomaNeuro-Oncology AdvancesAugust 4, 2021Craig Horbinski, Guifa Xi, Yufen Wang, Rintaro Hashizume, Mahesh Gopalakrishnan, Joanna J Phillips, Peter Houghton, Charles D James, John A KalapurakalFisher) using Bio-Rad imaging work station (Bio-Rad Laboratories). Antibodies specific for NF2/Merlin (#6995), RB (4H1, #9309), phospho-RB (p-RB, Ser780, #9307) were obtained from Cell Signaling Technologies.

(15) RAB11A-mediated YAP localization to adherens and tight junctions is essential for colonic epithelial integrityThe Journal of biological chemistryJuly 1, 2021Goswami S, Balasubramanian I, D'Agostino L, Bandyopadhyay S, Patel R, Avasthi S, Yu S, Goldenring JR, Bonder EM, Gao N.mouse anti-Merlin/NF2 (1:200; Abcam, ab217016), rabbit anti-Merlin/NF2 (D1D8) (1:400; Cell Signaling, 6995), rabbit anti-GRP78 (1:200; Abcam, ab21685), rabbit anti-GM130 (D6B1) (1:200; Cell Signaling, 12480),

(16) NF2 and Canonical Hippo-YAP Pathway Define Distinct Tumor Subsets Characterized by Different Immune Deficiency and Treatment Implications in Human Pleural MesotheliomaCancersMarch 29, 2021Yang H, Hall SRR, Sun B, Zhao L, Gao Y, Schmid RA, Tan ST, Peng RW, Yao F.(Cell Signaling Technology, #9153S), LATS2 (Novus Biologicals, #NBP3-03913), NF2, (Cell Signaling Technology, #6995S), beta-actin (Cell Signaling Technology, #3700S)) and anti-rabbit (#926–32211) or anti-mouse (#926-68020)

(17) Long Non-Coding RNA MAPK8IP1P2 Inhibits Lymphatic Metastasis of Thyroid Cancer by Activating Hippo Signaling via Sponging miR-146b-3pFrontiers in OncologyJanuary 7, 2021Xiaoli Liu et al.MST1 (#14946), p-LATS1(Thr1079) (#8654), LATS1 (#9153), p-YAP(Ser127) (#13008), YAP (#14074), NF2 (#6995), and RASSF1 (#86026) were purchased from Cell Signaling Technology, and TAZ from Abcam (ab224239),

(18) Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferationeLifeNovember 13, 2020Fomicheva M, Macara IG.acids 78–84) cloned into pWPI-mScarlet vector Antibody,Anti-NF2/MERLIN (rabbit monoclonal),CST,"CAT: 6995S, RRID:AB_10828709","(1:750), WB" Antibody,Anti-TRAF3 (mouse monoclonal),Santa Cruz,"CAT: sc-6933, RRID:AB_628390","(1:200),

(19) Adherens junction regulates cryptic lamellipodia formation for epithelial cell migrationThe Journal of cell biologyOctober 5, 2020Ozawa M, Hiver S, Yamamoto T, Shibata T, Upadhyayula S, Mimori-Kiyosue Y, Takeichi M.anti-phospho-Histone H3 (Ser10; Sigma-Aldrich; 06-570, 1:100 for IF); and rabbit anti-merlin antibody (Cell Signaling, D1D8; 6995, 1:100 for IF). Secondary antibodies used were Alexa Fluor 488 donkey anti-mouse IgG (Life Technologies;

(20) Yap/Taz promote the scavenging of extracellular nutrients through macropinocytosisGenes & developmentOctober 1, 2020King B, Araki J, Palm W, Thompson CB.(4856), S6 (2317), Erk1/2 pThr202/Tyr204 (4377), Erk1/2 (9107), Lats1 pT1079 (8654), Lats1 (3477), Nf2 (6995), Lamin A/C (4777), Histone H3 (3638), pan-TEAD (13295), Taz (4883), and human Axl (8661) were purchased

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Merlin (D1D8) Rabbit mAb

Merlin (D1D8) Rabbit mAb from Cell Signaling Technology

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Questions and Answers

Cell Signaling Technology

Citations (30)

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Merlin (D1D8) Rabbit mAb from Cell Signaling Technology

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