HIP2 (D27C4) Rabbit mAb 8226S from Cell Signaling Technology

Product Specs
ItemHIP2 (D27C4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8226S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk
TargetHIP2
HostRabbit
NCBI Gene AliasesE2-25K, HIP2, HYPG, LIG, UBC1
ClonalityMonoclonal
NCBI Full Gene Nameubiquitin conjugating enzyme E2 K
Research AreaUbiquitin and Ubiquitin-like proteins
IsotypeIgG
CloneD27C4
Molecular Weight25

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Targeting Colorectal Cancer Stem Cells Through Inhibition of the Fibroblast Growth Factor Receptor 4 Pathway with a Novel AntibodyCancers (Basel)January 28, 2026Gessica Filocamo, Mariachiara Buccarelli, Armin Lahm, Mirko Brunetti, Chantal Paolini, Gabriele De Luca, Michele Signore, Giorgia Castellani, Alessandra Boe, Romina Alfonsi, et al.anti-E-cadherin (rabbit mAb clone 24E10, # 3195, Cell Signaling, Danver, MA, USA), anti-beta Actin (mouse mAb #8226, Abcam, Cambridge, UK), and anti-GAPDH (mouse monoclonal G8795, Sigma-Aldrich, St. Louis, MO, USA).

(2) SIR-2.3/SIRT4 loss enhances proteostasis and neuronal resilience via AMPK-induced autophagy in Huntington’s disease modelsCell Communication and SignalingJanuary 15, 2026Cristina Trujillo-del Río, Seda Koyuncu, Julia Tortajada-Pérez, Mar Collado-Pérez, Ana Pilar Gómez-Escribano, Carlos Mora, Christian Neri, Agustín Lahoz, Marta Roca, José María Millán, et al.immunoblotting was performed with anti-HTT (Cell Signaling, ab#5656, 1:1,000), anti-β-actin (Abcam, #8226, 1:5,000), anti-LC3B (Cell Signaling Technology, 2775, 1:1,000) and anti-phospho-AMPKα (Thr172) (Cell Signaling Technology

(3) VDAC1 inhibitor DIDS uncouples respiration in isolated tissue mitochondria and induces mitochondrial hyperfusion in mammalian cellsBBA AdvOctober 8, 2025Surajit Das, Arpita Dutta, Minakshi Bedi, Arumay Pal, Shubhra Majumder, Alok Ghosh(1:500, Abclonal), rabbit anti-β Tubulin (2128T, 1:5000; Cell Signaling Technology), mouse anti-β-Actin (8226, 1:1000; Abcam). Secondary antibodies were either anti-mouse or anti-rabbit peroxidase-conjugated IgG

(4) Impaired PTEN Expression in T Cells Drives Resistance to Treg-Mediated Immune Regulation in Multiple SclerosisCellsSeptember 16, 2025Janine Schlöder, Bettina Trinschek, Felix Luessi, Helmut JonuleitGermany), mouse anti-PKB/Akt pSer437 mAb (11E6, Nanotools, Teningen, Germany), and mouse anti-β-Actin mAb (8226, Abcam, Cambridge, United Kingdom). Purified goat anti-rabbit IgG antibody (Cell Signaling Technology,

(5) The aging factor EPS8 induces disease-related protein aggregation through RAC signaling hyperactivationNature AgingSeptember 3, 2025Seda Koyuncu, Yaiza Dominguez-Canterla, Rafael Alis, Nassima Salarzai, Dunja Petrovic, Nuria Flames, David Vilchezimmunoblotting was conducted with anti-EPS8 (Proteintech, 12455-1-AP, 1:1,000), anti-β-actin (Abcam, 8226, 1:5,000), anti-HTT (Cell Signaling Technology, 5656, 1:1,000), FUS (Abcam, ab154141, clone CL0190,

(6) Suppression of NRAS-mutant melanoma growth with NRAS-targeting Antisense Oligonucleotide treatment reveals therapeutically relevant kinase co-dependenciesCommun Med (Lond)June 5, 2025Valentin Feichtenschlager, Yixuan James Zheng, Tiange Qu, Dasha Hohlova, Ciara Callanan, Linan Chen, Christopher Chen, Wilson Ho, Albert Lee, Yeonjoo Hwang, et al.dilution 1:600), B-ACTIN (Cell Signaling Technology®, cat.no.: 8457, dilution 1:3,000, or abcam, cat.no.:8226, dilution 1:1,000), p-Akt (Cell Signaling Technology®, cat.no.:4060, dilution 1:400), and Akt (Cell Signaling Technology

(7) Regulation of translation elongation and integrated stress response in heat-shocked neuronsCell ReportsMay 27, 2025Caitlin M. Seluzicki, Milad Razavi-Mohseni, Fulya Türker, Priyal Patel, Boyang Hua, Michael A. Beer, Loyal Goff, Seth S. Margolisproteasome subunit (MCP231) (1:1000) (Enzo life sciences, BML-PW8195); Anti-actin (1:2000) (Abcam, 8226); Anti-caspase 3 (1:1000) (Cell Signaling Technology, 9662); Anti-phosphorylated eEF2 (1:500, in 5%

(8) YY1-induced transcription of AKR1C3 activates the Hedgehog signalling pathway to enhance lenalidomide resistance and glycolytic activity in multiple myeloma cellsClin Exp MedMarch 29, 2025Yang Chen, Aijia Zhang, Yuan Wang, Daoda Qi, Chengyi Peng, Zihao Liang, Jingjing Guo, Yan GuTarget,Catalogue,Company AKR1C3,"PA5-28,065",Thermofisher HK2,#2867,CST GLUT1,"#MA5-31,960",Thermofisher LDHA,"#PA5-27,406",Thermofisher GLI1,#2553,CST PTCH1,#2468,CST HIP1,#8226,CST YY1,ab109237,abcam ACTB,#MA1-140,Thermofisher gamma-H2AX,A700-053,Thermofisher CEBPA,ab317442,abcam PKM2,#4053,CST ATF4,Ab85049,abcam Antibodies

(9) Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilinFrontiers in PhysiologyMarch 18, 2025Patricia E. McCallinhart, Kathlyene R. Stone, Pamela A. Lucchesi, Aaron J. Trask(concentration 1:1000) was acquired from Cell Signaling Technology for Western blot analysis. Beta actin (8226) (concentration 1:1000) was obtained from Abcam for Western blot analysis. Cofilin (PA5-27627) for staining

(10) Cellular adaptation to cancer therapy along a resistance continuumNatureJuly 1, 2024França GS, Baron M, King BR, Bossowski JP, Bjornberg A, Pour M, Rao A, Patel AS, Misirlioglu S, Barkley D, et al.quantification was performed using ImageJ software. Anti-beta actin antibody (Santa Cruz, sc-47778 or mAbcam 8226) was used as a loading control for all of the following staining, the NRF2 (Cell Signaling Technology

(11) Gingival-derived mesenchymal stem cells alleviate allergic asthma inflammation via HGF in animal modelsiScienceMay 17, 2024Fang Q, Wu W, Xiao Z, Zeng D, Liang R, Wang J, Yuan J, Su W, Xu X, Zheng Y, et al.D3Z2G",CST,"Cat#12640s, RRID:AB_2629499" "Mouse Anti-Actin, beta Monoclonal Antibody, Clone mAbcam 8226",abcam,"Cat#Ab8226, RRID:AB_306371" HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L),BOSTER,"Cat#BA1054,

(12) LncRNA AGAP2 antisense RNA 1 stabilized by insulin-like growth factor 2 mRNA binding protein 3 promotes macrophage M2 polarization in clear cell renal cell carcinoma through regulation of the microRNA-9-5p/THBS2/PI3K-Akt pathwayCancer Cell IntDecember 18, 2023Peng Xu, Da-Xiong Feng, Jun Wang, Yao-Dong Wang, Gang Xie, Bin Zhang, Xiao-Han Li, Jia-Wei Zeng, Jia-Fu FengUSA). After that, the membrane was incubated with primary antibodies, including mouse anti-β-actin (#8226, 1:5000, CST, US), rabbit anti-THBS2 (ab112543, 1:1000, Abcam, UK), rabbit anti-PI3K (#4292, 1: 1000,

(13) Extracellular vesicles derived from immortalized human natural killer cell line NK3.3 as a novel therapeutic for multiple myelomaFrontiers in ImmunologySeptember 25, 2023Emily C. Matchett, Jacki KornbluthIgG (#7076 and #7074, Cell Signaling Technology). 2.8 Calcein AM release cytotoxicity assays K562, 8226/Luc, and U266 cells were co-cultured with NK3.3 and NK3.3-LTV cells to test cytotoxicity of NK3.3-LTV

(14) Biallelic DDHD2 mutations in patients with adult‐onset complex hereditary spastic paraplegiaAnnals of Clinical and Translational NeurologySeptember 1, 2023Ying‐Tsen Chou, Shao‐Lun Hsu, Yu‐Shuen Tsai, Yi‐Jiun Lu, Kai‐Wei Yu, Hsiu‐Mei Wu, Yi‐Chu Liao, Yi‐Chung LeeDanvers, MA, USA). β‐Actin was used as a loading control and detected with anti‐β‐actin antibody (#8226; Abcam, Cambridge, UK). To determine the stability of the WT and mutant DDHD2 proteins, CHX‐chase assays

(15) MAP4K3 inhibits Sirtuin-1 to repress the LKB1-AMPK pathway to promote amino acid-dependent activation of the mTORC1 complexLife science allianceAugust 1, 2023Branch MR, Hsu CL, Ohnishi K, Shen WC, Lee E, Meisenhelder J, Winborn B, Sopher BL, Taylor JP, Hunter T, et al.(M2),F1804,Sigma-Aldrich Phospho-PKC-θ (T583),9377,Cell Signaling PKC-θ (E117Y),13643,Cell Signaling β-actin,8226,Abcam α-Tubulin,62204,Thermo Fisher Scientific F-actin,A22287,Invitrogen List of antibodies.

(16) Triple combination of lomustine, temozolomide and irradiation reduces canine glioma cell survival in vitroVeterinary Medicine and ScienceJuly 1, 2023Daniel Fuchs, Carla Rohrer Bley, Luca Morandi, Caterina Tonon, Mathias S. Weyland, Katarzyna J. Nytkoanti‐mouse IgG, HRP‐linked secondary antibody (#7076S, 1:1000, Cell Signaling). For loading control, a β‐actin antibody (8226, 1:1000, Abcam) was used. Pierce™ ECL Western Blotting Substrate (Thermo Fischer Scientific, Waltham,

(17) Lithium chloride promotes diabetic corneal epithelial wound healing by activating the Wnt/β‑catenin signaling pathwayExperimental and Therapeutic MedicineJune 20, 2023Jing Dan, Ting Tan, Man Wu, Jin Gong, Qingguo Yang, Linling Wang, Ping Wang(dilution 1:1,000; 12456, Cell Signaling Technology, Danvers, USA) and β-actin (dilution 1:1,000; cat. no. 8226; Abcam). On the second day, the membrane was incubated with a goat anti-rabbit IgG H&L HRP-coupled secondary

(18) Hypoxia regulates adipose mesenchymal stem cells proliferation, migration, and nucleus pulposus-like differentiation by regulating endoplasmic reticulum stress via the HIF-1α pathwayJournal of orthopaedic surgery and researchMay 8, 2023Wu J, Yu L, Liu Y, Xiao B, Ye X, Zhao H, Xi Y, Shi Z, Wang W.(ab185966, Abcam, USA), CHOP(2895,CST), ATF6 (1-40,256, NBP), and ERK (5683p, CST) and β-actin (ab 8226, Abcam, USA). The membrane was washed and incubated with secondary antibodies (Beyotime, Shanghai, China)

(19) UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinomaInternational journal of oncologyApril 1, 2023Fu W, Lei X, Lu Q, Zhang J, Guo J, Zhao J, Tong X, Hu X.(Proteintech Group, Inc.; cat. no. 11834-3-AP, 1:2,000. CST Biological Reagents Co., Ltd.; cat. no. 8226; 1:1,000), anti-IGF2BP3 (Proteintech Group, Inc.; cat. no. 14642-1-AP; 1:3,000), anti-NANOG (CST Biological

(20) Trispecific T-cell engagers for dual tumor-targeting of colorectal cancerOncoImmunologyDecember 31, 2022Antonio Tapia-Galisteo et al.phospho-EGFR (Tyr1068) clone D7A5 (cat#3777, Cell Signaling Technology Inc) and anti-β-actin mouse clone 8226 (cat#ab8226, Abcam). The polyclonal antibodies included: PE-conjugated goat F(ab’)2 fragment anti-mouse

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HIP2 (D27C4) Rabbit mAb

HIP2 (D27C4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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HIP2 (D27C4) Rabbit mAb from Cell Signaling Technology

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