Phospho-IkappaBalpha (Ser32) (14D4) Rabbit mAb (Biotinylated) 5209S from Cell Signaling Technology

Product Specs
ItemPhospho-IkappaBalpha (Ser32) (14D4) Rabbit mAb (Biotinylated)
CompanyCell Signaling Technology
Price $477.00 Inquire
Catalog Number5209S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetIkB-a (Ser32) phosphate
ModificationsIkB-a (Ser32) phosphate
HostRabbit
NCBI Gene AliasesEDAID2, IKBA, MAD-3, NFKBI
Conjugate/TagBiotin
ClonalityMonoclonal
NCBI Full Gene NameNFKB inhibitor alpha
Research AreaImmunology and Inflammation
IsotypeIgG
Clone14D4
Molecular Weight40

Questions and Answers

What species does this antibody detect, and has it been used in relevant studies?

The Phospho-IκBα (Ser32) Rabbit mAb (Biotinylated) detects endogenous levels of IκBα only when phosphorylated at Ser32 and is reactive with mouse, rat, non-human primate, and human samples. It has been cited in multiple studies, including those investigating the NF-κB signaling pathway in various contexts, such as inflammation and cancer.

Can this antibody be used in Western blotting, and what has been documented regarding its application?

Yes, this antibody is suitable for use in Western blotting. It has been utilized in studies to assess the phosphorylation status of IκBα, which is crucial for understanding NF-κB signaling dynamics in various biological processes, including inflammation and cancer progression.

Are there specific experimental contexts where this antibody has been effectively applied?

This antibody has been effectively applied in studies examining the NF-κB pathway, such as in the context of osteoclastogenesis and inflammation, as demonstrated in research exploring the effects of various compounds on NF-κB activation and its downstream effects on cellular processes.

What modifications does this antibody specifically recognize, and why is this important for experimental design?

This antibody specifically recognizes phosphorylated IκBα at Ser32. This specificity is important for experimental design as it allows researchers to accurately assess the activation state of the NF-κB pathway, which is critical for understanding its role in various cellular responses, including immune responses and tumorigenesis.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Thlaspi arvense attenuates colitis-associated colorectal tumorigenesis through suppression of neutrophil recruitment via the NOD/NF-κB pathwayChin MedApril 17, 2026Ziwei Wang, Wenkai Wang, Chaowei Wang, Bijin Dong, Yunchuan Sun, Xinying He, Ling Bi, Yan WangGAPDH (2118S), NF-κB p65 (8242S), Phospho-NF-κB p65 (Ser536, 3033S), IκBα (4812S), phospho-IκBα (Ser32, 5209S), Ki-67 (34330SF), and Ly-6G (1A8) rat mAb (88876S) from Cell Signaling Technology (Massachusetts, USA);

(2) Interaction of coronavirus E protein with BRD2 plays important regulatory roles in viral replication and induction of pro-inflammatory responseJ VirolMarch 3, 2026Shumin Li, Ding Xiang Liureagents Antibodies against BRD2 (#5848), NF-κB p65 (#8242), Phospho-NF-κB p65 (#3033), IκBα (#4814), Phospho-IκBα (#5209), IL-6 (#12912), FLAG-Tag (#8146), GFP-Tag (#2956), myc-Tag (#2276), H3 (#4499), α-Tubulin (#3873),

(3) EPHA5 regulates antifungal innate immunity by phosphorylating EPHB2 and Dectin-1PLoS PathogJune 9, 2025Ru Gao, Heping Wang, Zhihui Cui, Yanyun Du, Ruirui He, Lingyun Feng, Bo Zeng, Yangyang Li, Guoling Huang, Ting Pan, et al.(Thr183/Tyr185), anti-JNK1, anti-p-Syk, and anti-EPHB2, all of which were purchased from CST (cat. no. 5209S, 4668S, 3708S, 2710S, and 83029S). Anti-EPHA5, anti-Flag, and anti-HA antibodies were obtained from

(4) Regulator of G protein signaling-1 facilitates ovarian cancer development by modulating NF-kB signal pathwaySci RepJanuary 5, 2025Liu Shengnan, Xi Jiayan, Sun Meng, Li Li, Cai Shengyun, Xu Mingjuan(1:1000, #4138), cyclinD1 (1:1000, #2922), p21 (1:1000, #2946), p-p65 (1:1000, #3033), p-IκBα (1:1000, #5209), and GAPDH (1:2000, #3683) antibodies. IκBα (1:1000, 10268-1-AP) and p65 (1:1000, CL594-10745) were

(5) Exploring the molecular mechanism of Yinao Fujian formula on ischemic stroke based on network pharmacology and experimental verificationHeliyonJanuary 15, 2024Jing Lu, Xiaolei Tang, Yuxin Zhang, Hongbo Chu, Chenxu Jing, Yufeng Wang, Huijuan Lou, Ziqi Zhu, Daqing Zhao, Liwei Sun, et al.Abcam (Cambridge). Antibodies against p-p65 (65 kDa, #3033), p65 (65 kDa, #8242), p-IκBα (40 kDa, #5209), Keap-1 (60–64 kDa, #8047), HO-1 (28 kDa, #43966) were obtained from Cell Signaling Technology (Beverly,

(6) Acacetin inhibits inflammation by blocking MAPK/NF-κB pathways and NLRP3 inflammasome activationFrontiers in pharmacologyJanuary 1, 2024Bu J, Mahan Y, Zhang S, Wu X, Zhang X, Zhou L, Zhang Y.p65,ab76302,1:800,60,"abcam, United Kingdom" IκBα,ab32518,1:1000,60,"abcam, United Kingdom" Phospho-IκBα,5209s,1:800,60,"CST, United States" ERK,ab184699,1:800,60,"abcam, United Kingdom" Phospho-ERK,ab201015,1:800,60,"abcam,

(7) A novel FGFR1 inhibitor CYY292 suppresses tumor progression, invasion, and metastasis of glioblastoma by inhibiting the Akt/GSK3β/snail signaling axisGenes & diseasesJanuary 1, 2024Bi Y, Zheng R, Hu J, Shi R, Shi J, Wang Y, Wang P, Jiang W, Kim G, Liu Z, et al.#4903.1:1000), anti-IκBɑ (Cell Signaling, #9242.1:2000), anti-phosphorylated IκBɑ (Cell Signaling, #5209.1:2000), anti-p65 (Cell Signaling, #8242, 1:500), anti-phosphorylated p65 (Cell Signaling, #13346,1:500),

(8) JianPi-QingHua formula attenuates nonalcoholic fatty liver disease by regulating the AMPK/SIRT1/NF-κB pathway in high-fat-diet-fed C57BL/6 micePharmaceutical BiologyDecember 31, 2023Jing Tian, Mengjie Cai, Shenyi Jin, Qingguang Chen, Jiahui Xu, Qiuyue Guo, Zihui Yan, Xu Han, Hao Lu(5832S), p-ACC (3661S), ACC (3676S), SIRT1 (8469S), p-IKK (2697S), IKKα (2682S), IKKβ (8943S), p-IκBα (5209S), IκBα (4812S), p-NF-κB p65 (3033S), NF-κB p65 (8242S), F4/80 (70076S). GAPDH (10494-1-AP, Proteintech,

(9) Atsttrin regulates osteoblastogenesis and osteoclastogenesis through the TNFR pathwayCommun BiolDecember 11, 2023Kaiwen Liu, Zihao Wang, Jinbo Liu, Wei Zhao, Fei Qiao, Qiting He, Jie Shi, Qunbo Meng, Jianlu Wei, Lei Cheng(#4695), anti-p-Erk1/2 (#8544),anti-AKT (#4691),anti-p-AKT (#4060),anti-IκBα (#4814), anti-p-IκBα (#5209), anti-p-P65 (#3033), and anti-P65 (#8242) from Cell Signaling Technology (Danvers, MA, USA). We have

(10) The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathwayVeterinary ResearchNovember 30, 2023Xiao Liu, Yanan Zhu, Dan Wang, Ran Feng, Zhihao Chen, Zifang Zheng, Yang Li, Lele Xu, Haixue Zheng, Yunpeng Fan, et al.ABclonal Technology. The anti-p-TRAF2 (13908), anti-P65 (8242), anti-p-P65 (3033), anti-IκBα (9242), and anti-p-IκBα (5209) antibodies were purchased from Cell Signaling Technology. Lipofectamine™ RNAiMAX Transfection Reagent

(11) PFKP confers chemoresistance by upregulating ABCC2 transporter in non-small cell lung cancerTranslational Lung Cancer ResearchNovember 30, 2023Zimin Wang, Sikai Wu, Xin Chen, Jiawei Liang, Fenglai Xue, William C. Cho, Jiaqian Bao, Baofu Chen, Minhua Ye, Dehua Ma, et al.MatrigengeL Matrix (Cat 0827555) was from ABW (Shanghai, China). γ-H2AX (Ser139) (Cat 2577), p-IκBα (Cat 5209), IκBα (Cat 4814), p-p65 (Cat 3033) and p65 (Cat 8242T) were brought from Cell Signaling Technology

(12) Deubiquitinase OTUD6A Regulates Innate Immune Response via Targeting UBC13VirusesAugust 18, 2023Li Z, Li G, Li Y, Luo Y, Jiang Y, Zhang Z, Zhou Z, Liu S, Wu C, You F.8242); Anti-p-p65 (Ser536) (93H1) (CST, Cat# 3033); Anti-IκBα (CST, Cat# 9242); Anti-p-IκBα (CST, Cat# 5209); Goat anti-mouse IgG, HRP conjugate (Proteintech, Cat# SA00001-1); and Goat anti-rabbit IgG, HRP conjugate

(13) IGJ suppresses breast cancer growth and metastasis by inhibiting EMT via the NF‑κB signaling pathwayInternational Journal of OncologyJuly 31, 2023Mengxue Wang, Yushen Wu, Xunjia Li, Meng Dai, Shengwei Li(p-)-p65 (Ser536; 1:1,000; cat. no. 3033), IκBα (1:1,000; cat. no. 4818), p-IκBα (Ser32; 1:1,000; cat. no. 5209), proliferating cell nuclear antigen (PCNA; 1:1,000; cat. no. 13110), and GAPDH (1:1,000; cat. no. 51332)

(14) Inhibiting NFAT5 With KRN2 Mitigates Acute Allograft Rejection in a Murine Heart Transplantation ModelJournal of Cardiovascular PharmacologyMarch 1, 2023Chenghao Li, Xing Chen, Yixuan Wang, Yajun Huang, Guohua Wangsignaling technology [CST]), NF-kB p65 (diluted at 1:1,000, 8242T, CST), phosphoIkBa (diluted at 1:500, 5209S, CST), IkBa (diluted at 1:500, 4812S, CST), and glyceraldehyde-3-phosphate dehydrogenase (diluted at

(15) Inhibiting NFAT5 With KRN2 Mitigates Acute Allograft Rejection in a Murine Heart Transplantation ModelJ Cardiovasc PharmacolDecember 22, 2022Chenghao Li, Xing Chen, Yixuan Wang, Yajun Huang, Guohua Wangsignaling technology [CST]), NF-kB p65 (diluted at 1:1,000, 8242T, CST), phosphoIkBa (diluted at 1:500, 5209S, CST), IkBa (diluted at 1:500, 4812S, CST), and glyceraldehyde-3-phosphate dehydrogenase (diluted at

(16) Mesenchymal stem cell-derived extracellular vesicles protect retina in a mouse model of retinitis pigmentosa by anti-inflammation through miR-146a-Nr4a3 axisStem cell research & therapyAugust 3, 2022Zhang J, Li P, Zhao G, He S, Xu D, Jiang W, Peng Q, Li Z, Xie Z, Zhang H, et al.CST, #12,242), mouse anti-IκBα (1:1000, CST, #4814), rabbit anti-Phospho-IκBα (Ser32) (1:1000, CST, #5209), mouse anti-NF-κB p65 (1:1000, CST, #8242), rabbit anti-CD9(1:1000, CST, #13403S), rabbit anti-CD63

(17) A novel ribosomal protein S6 kinase 2 inhibitor attenuates the malignant phenotype of cutaneous malignant melanoma cells by inducing cell cycle arrest and apoptosisBioengineeredMay 1, 2022Li Y, Yu P, Long J, Tang L, Zhang X, Zhou Z, Cao D, Su J, Chen X, Peng C.(Santa Cruz Biotechnology, TX, USA, #sc-245, 1:1000), p-IκBα (Cell Signaling Technology, MA, USA, #5209S, 1:1000), GAPDH (Proteintech, MA, USA, #60004-1-Ig, 1:1000). Pull down assay Cell lysates were incubated

(18) MiR-92a/KLF4/p110δ regulates titanium particles-induced macrophages inflammation and osteolysisCell death discoveryApril 13, 2022Wen Z, Lin S, Li C, Ouyang Z, Chen Z, Li S, Huang Y, Luo W, Zheng Z, Guo P, et al.for WB and IF assays), phospho-IKKα/β (#2694, for WB assay), IKKβ (#8943, for WB assay), phospho-IκBα (#5209, for WB assay), and IκBα (#4812, for WB assay) were purchased from Cell Signaling Technology (Massachusetts,

(19) The protective effects of Olmesartan against interleukin-29 (IL-29)-induced type 2 collagen degradation in human chondrocytesBioengineeredJanuary 11, 2022Yunlong Liu, Junyi Liu, Yan Ma, Yongyong Zhang, Qiong Chen, Xin Yang, Yanchun ShangCell Signaling Technologies, USA), IκBα (1:3000; #4814, Cell Signaling Technologies, USA), p-IκBα (1:1000, #5209, Cell Signaling Technologies, USA), NF-κB p65 (1:2000; #3036, Cell Signaling Technologies, USA), lamin

(20) Interleukin 10 Attenuates Angiotensin II-Induced Aortic Remodelling by Inhibiting Oxidative Stress-Induced Activation of the Vascular p38 and NF-κB PathwaysOxidative medicine and cellular longevityJanuary 1, 2022Qiu M, Shu H, Li L, Shen Y, Tian Y, Ji Y, Sun W, Lu Y, Kong X.phospho-p38 MAPK (Thr180/Tyr182, cat. no. 4511), p38 (cat. no. 8690), phospho-IκBα (Ser32) (cat. no. 5209), IκBα (cat. no. 4814), IKKα (cat. no. 2682), IKKβ (cat. no. 2678), and phospho-IKKα/β (Ser176/180,

Be the First to Write a Review!

Phospho-IkappaBalpha (Ser32) (14D4) Rabbit mAb (Biotinylated)

Phospho-IkappaBalpha (Ser32) (14D4) Rabbit mAb (Biotinylated) from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (52)

Reviews

Phospho-IkappaBalpha (Ser32) (14D4) Rabbit mAb (Biotinylated) from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map