Immunocytochemistry/Immunofluorescence analysis of RHD in K562 cells using RHD Chimeric Monoclonal Antibody (Product # MA5-48188). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with RHD Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom RHD Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Mouse IgG1 Isotype Control Antibody (Product # MA5-47823) followed by staining with Alexa Fluor 488 secondary antibody.