Immunocytochemistry/Immunofluorescence analysis of CD25 in mouse splenocytes using CD25 Chimeric Monoclonal Antibody (Product # MA5-48353). Paraformaldehyde-fixed cells were immobilized on a coverslip and stained with CD25 Chimeric Monoclonal Antibody at a 1:100 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom CD25 Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was a Rabbit IgG Chimeric Monoclonal Antibody (Product # MA5-47825) followed by staining with Alexa Fluor 488 secondary antibody.