Immunocytochemistry/Immunofluorescence analysis of CD25 in Jurkat cells using CD25 Chimeric Monoclonal Antibody (Product # MA5-48112). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CD25 Chimeric Monoclonal Antibody at 10 µg/mL followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom CD25 Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was stained with FITC Chimeric Monoclonal Antibody (Product # MA5-47752) followed by Alexa Fluor 488 secondary antibody.