Fig 1: OGT is upregulated in ccRCC and associated with cancer progression(A and B) The protein (A) and mRNA (B) expression of OGT, OGA, and O-GlcNac in ccRCC and paired non-tumor renal tissues (n = 5).(C) The expression of OGT in different grades of ccRCC (Grade 1: n = 36, Grade 2: n = 42, Grade 3: n = 10, Grade 4: n = 4) detected by IHC staining.(D) The expression of OGT in different pathological stages of ccRCC (Stage Ⅰ: n = 72, Stage Ⅱ: n = 6, Stage Ⅲ: n = 14) detected by IHC staining.(E) The association between OGT and overall survival of 512 cases patients with ccRCC in the TCGA database (Kaplan-Meier analysis).(F–H) The association between OGT (F), OGA (G), O-GlcNac (H) and overall survival of our 92 cases of patients with ccRCC (Kaplan-Meier analysis). Scale bar, 400 μm. Data are represented as mean ± SD. Statistical analysis of the data from 2 groups was performed using Student’s t test. Comparisons among multiple groups were performed by one-way ANOVA followed by Fisher’s LSD test. (∗p < 0.05, ∗∗∗p < 0.001).