Fig 1: bFGF-Chitosan gel induces migration of neural precursors derived from Nestin lineage toward peri-infract cortex.(A) Schematic diagram of NestinCreERT2-tdTomato transgenic mice. (B) Representative immunofluorescence images showed tdTomato + cells express Nestin in SVZ (tdTomato/Nestin/topro). (C) Representative immunofluorescence images from peri-infarct cortex of tdTomato + cells with various neuronal progenitor markers CD133, SOX2, and MASH1 on day 14 post-stroke. (D) Schematic of differentiation stages as defined by marker expression. Neural stem cells (NSC) produce neuronal progenitor cells (NPCs), which give rise to neurons. (E) Quantitative analyses of cell density of tdTomato+, tdTomato+/CD133+, tdTomato+/SOX2+, and tdTomato+/MASH1+ in peri-infarct cortex on day 14 post-stroke. ∗P < 0.05, ∗∗P < 0.01, independent-samples T test, n = 4 in each group. (F) Quantitative analyses of differential rate of NPCs among tdTomato + cells on day 14 post-stroke. Independent-samples T test, n = 4 in each group. Schematic diagram was created with BioRender.com.