Fig 2: Overexpression of CAPRIN1 counteracts the inhibition of OC cellular behaviors caused by NAT10 knockdown. A CAPRIN1 mRNA expression and (B) protein levels in OC cells (CaOV3, A2780, and SKOV3) and normal IOSE-80 cells were examined using qPCR and immunoblotting, respectively. C CAPRIN1 expression in CaOV3 and SKOV3 cells following empty vector and CAPRIN1 overexpressing plasmids transfection. After NAT10 knockdown and CAPRIN1 overexpression, (D) cell migration was evaluated using Transwell assay, and (E) migrated cells were counted; (F) invasion was evaluated using Transwell assay, and (G) invaded cells were counter; (H, I) sphere formation analysis was used to assess stemness; and (J) immunoblotting was conducted to examine the levels of stemness-related factors, including Nanog, OCT4, and SOX2

Fig 3: NAT10 facilitates ac4C modification of CAPRIN1. A NAT10-associated genes were predicted using the LinkedOmics database through Pearson correlation coefficient. Correlation between NAT10 and (B) TRIM44, (C) CAPRIN1, and (D) QSER1 was predicted using the GEPIA database. The expression of (E) TRIM44, (F) CAPRIN1, and (G) QSER1 was measured using qPCR after NAT10 knockdown. H The regulation of NAT10 knockdown on ac4C levels of CAPRIN1 was measured using ac4C-RIP assay. I The relation of NAT10 and CAPRIN1 was evaluated using RIP. J A representative photograph of electrophoresis results in RIP. K Effect of NAT10 on CAPRIN1 mRNA stability was measured using actinomycin D treatment assay. NS: no significance