Fig 1: miR-378 overexpression promotes lipophagy in hepatocytes. (a) Immunofluorescence staining of LC3 in AML12 cells transfected with miR-378 mimics under HBSS starvation conditions. (b) Immunofluorescence staining of LC3 in AML12 cells transfected with miR-378 in the absence or presence of Baf-A1. (c) Western blot analysis of LC3 in HepG2 cells transfected with miR-378 mimics in the absence or presence of Baf-A1. (d) Representative images of AML12 cells stably expressing mRFP-GFP-LC3 infected with Ad-378 and following HBSS treatment. AML12 cells stably expressing the autophagy reporter mRFP-GFP-LC3 were infected with Ad-378, followed by HBSS treatment for 2 h. (e) BODIPY staining of HepG2 cells transfected with miR-378 mimics co-treated with or without PA followed by HBSS treatment. (f and g) Immunofluorescence staining for BODIPY (593/403) and LC3 (f) or LAMP2 (g) in PA-pretreated AML12 cells infected with Ad-378, followed by HBSS treatment.