Fig 1: ISG mRNAs evade RNase L-mediated RNA decay, permitting their translation(A) Quantitative proteomic analyses in WT and RL-KO cells.(B) Heatmap showing the induction of immediate-early and ISGs in response to poly(I:C).(C) Heatmap showing the effect of RNase L from (B).(D) Western blot analysis of ISG proteins in WT and RL-KO at 0, 8, and 16 h post-lipofection of poly(I:C).(E) smRNA-FISH for the indicated ISG mRNAs and GAPDH mRNA under mock conditions or 8 h post-lipofection of poly(I:C). Scale bar, 10 μm.(F) Quantification of smRNA-FISH puncta as represented in (E). Each dot represents a single cell. n = 30 or more cells.(G) smRNA-FISH for OASL mRNA and GAPDH mRNAs and IFA for OASL protein. White arrows indicate cells with degraded GAPDH mRNA, yellow arrows indicate cells with GAPDH mRNA. Scale bar, 10 μm.(H) Quantification of smRNA-FISH puncta and mean fluorescence intensity (MFI) for OASL protein in individual cells (dots) as represented in (G). n = 36 cells. Significance was determined by Student’s t test (Excel). *p < 0.05, **p < 0.01, and ***p < 0.001.