Fig 2: MI Gel Restored the Intestinal Barrier in CRF Mice. (A) Colon length statistics and the according macroscopic appearance; (B) Representative micrographs of H&E staining and immunohistochemical detection of ZO-1 and Occludin in colon tissues; (C-F) mRNA expression levels of ZO-1, Occludin, TNF-α, and IL-6 in colon tissue; (G-H) Representative fluorescence images of WGA and ROS staining in colon sections; (I-J) mRNA expression levels of IL-1β and iNOS in colon tissue. * p < 0.05, ** p < 0.01, *** p < 0.001

Fig 3: MI Gel Ameliorates Intestinal Environment in IBD Mice. (A) Establishment and treatment regimen of IBD mouse model; (B) Body weight change rate; (C) DAI scores across groups; (D) Observation of lower gastrointestinal bleeding after treatment; (E) H&E staining of mouse colon sections; (F) Representative macroscopic images and length statistics of colorectum; (G-I) Serum levels of TNF-α, IL-6, and IL-1β; (J-N) mRNA levels of IL-1β, TNF-α, IL-6, iNOS, and Occludin in intestinal tissue; (O) Schematic diagram of MI Gel improve intestinal inflammation. * p < 0.05, ** p < 0.01, *** p < 0.001