Fig 1: Western blot and densitometry results for NRP1 (A,B), SDC2 (C,D), and GPC4 (E,F) from NE-targeted BMDM lysates. Three separate blood monocyte donations were obtained from healthy donors and cultured in RPMI with GM-CSF for 8–10 days to differentiate cells to blood monocyte derived macrophages. Cells from each donor were treated with control vehicle or NE (200 or 500 nM) for 1 or 2 h. Cell lysates were collected, protein quantified, and Western analyses were performed for NRP1 (A), SDC2 (C), and GPC4 (E). Left panels are representative Western blots for protein targets and as a control, β-actin. After densitometry of bands using ImageJ, relative expression corrected for β-actin was compared to control treated samples and summary data of relative expression for each protein shown in the panel on the right, NRP1 (B), SDC2 (D), and GPC4 (F). Significant differences in relative expression for N = 3 per protein was determined; *, p < 0.05; **, p < 0.01; ***, p < 0.001.