Fig 2: GRB7 partially rescues the effect of downregulation of TCF12 in HER2+ cells. A MTT assay, B colony formation assay and C EdU assay showed that GRB7 could partially reverse the inhibitory effect of TCF12 knockdown on cell proliferation. D Wound healing assays and E Transwell assays indicated that overexpression of GRB7 partially restored the inhibitory effect of TCF12 downregulation on cell migration and invasion. Data are shown as the mean ± SD, N ≥ 3. *p < 0.05, **p < 0.01, ***p < 0.001

Fig 3: GRB7 promotes HER2 progression in vivo by increasing TCF12-induced EMT reduction. A Tumor growth curve of xenograft tumors from the indicated groups. B Representative images of xenograft tumors from the indicated groups. C Tumor weights from the indicated groups. D, E EMT-related protein expression was analyzed by IHC and western blot in tumor samples from the indicated groups. Data are shown as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001. F Schematic of the molecular mechanism by which GRB7 is transcriptionally regulated by TCF12 to regulate HER2+ BC progression through the Notch/AKT/ERK axis