Fig 1: NLRP1 and NLRP3 are dispensable for VBP and MeBs responses in THP1 monocytes.THP1 monocytes expressing Cas9 were electroporated with indicated sgRNAs (TRAC was used as control). a) IL-18 ELISA, b) LDH release assay, c) immunoblot and d) IL-1β cytometric bead array. c) Immunoblot was used to confirm knock-out efficiency. Non-specific (*) and bands of interest (arrowhead) are indicated. e) Immunoblot of cleaved-GSDMD of indicated treatments. Experiments are representative of at least three independent experiments. Mean is shown with standard deviation of biological replicates corresponding to independently treated wells (n=3). Two-way ANOVA, Tukey’s multiple comparison test. ****p<0.0001, ***p<0.001. Valboro-pro (VBP) 25 μM, 10 μM methyl ester bestatin (MeBs) treated 24h.