Published customer image:
Rat anti Human CD195 antibody, clone HEK/1/85a (MCA2175) used for the immunoprecipitation of CD195 from transfected CHO lysates.
Image caption:
Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.

From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10(4): e0125396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Published customer image:
Rat anti Human CD195 antibody, clone HEK/1/85a (MCA2175) used for the immunoprecipitation of CD195 from transfected CHO lysates.
Image caption:
Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.

From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10(4): e0125396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Published customer image:
Rat anti Human CD195 antibody, clone HEK/1/85a (MCA2175) used for the immunoprecipitation of CD195 from transfected CHO lysates.
Image caption:
Ligand-elicited recruitment of arrestin2 to CCR5. CHO-CCR5 cells stably transfected with arrestin2-GFP were treated with the indicated chemokines (100 nM, 50 min), washed extensively, crosslinked, washed again and lysed prior to immunoprecipitation with an anti-CCR5 antibody. Immunoprecipitates were subjected to Western blot using antibodies against GFP, arrestin2 and CCR5. CP; total cell pellet control, IP; antibody specificity used for immunoprecipitation, IB; antibody specificity used for immunoblot.

From: Bönsch C, Munteanu M, Rossitto-Borlat I, Fürstenberg A, Hartley O (2015)
Potent Anti-HIV Chemokine Analogs Direct Post-Endocytic Sorting of CCR5. PLoS ONE 10(4): e0125396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.