Western Blot: UNG Antibody [NB600-1031]
UNG Antibody
Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for UNG Antibody - BSA Free
Available conjugates: Available conjugates: UnconjugatedSpecificity: The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus the antibody recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kDa, 27.5 kDa, 31 kDa, and 38 kDa forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).Sizes Available: 0.1 mg (also 0.025 mg)