MyBioSource.com's Rabbit Anti-Histone H3 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot. The Rabbit Anti-Histone H3 Antibody was generated using H3 clustered histone 1, and H3C1 as the antigen and it reacts with Human, Mouse, and Rat.
Description
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin. Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated. Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. This antibody is specific for histone H3 Di-methylated at K9. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the Dimethylation modification is present