MyBioSource.com's Anti-MAGEA3 [21B4], Rabbit IgG is a Rabbit monoclonal antibody. The Anti-MAGEA3 [21B4], Rabbit IgG Antibody was generated using MAGEA3 as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
The binding specificity of this antibody towards MAGE-A3 was determined using ELISA. The original antibody cross reacts with MAGEA6 but does not react with other members of the MAGE family in an ELISA or western blot assay. This antibody was used in the detection of HEK293T cells expressing MAGEA3 and MAGEA6 using flow cytometry. The original IgG1 antibody was capable of immunoprecipitating MAGE-A3 from lysates of SK-MEL-37 cells endogenously expressing MAGEA3. The original antibody was also used in the immunohistochemical staining of tissues form normal testis and squamous cell carcinoma of the lung (US20140093514)