Western Blot: ING4 Antibody [NB100-1682]
Western Blot: ING4 Antibody [NB100-1682] - Used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at 29 kDa corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.
ING4 Antibody
Supplier Page from Novus Biologicals, a Bio-Techne Brand for ING4 Antibody - BSA Free
Available conjugates: Available conjugates: UnconjugatedSpecificity: Reactivity occurs against human p29 ING4 protein. The sequence is not present in isoforms 2 (see below). However, 100% homology is on record for this protein from human, dog, rat, and mouse. Cross reactivity with p29 ING4 protein from chimpanzee and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p29 ING4 proteins from other sources is not known.Sizes Available: 0.1 mg