CD31/PECAM-1 Antibody (MEC 7.46)

Product Specs
ItemCD31/PECAM-1 Antibody (MEC 7.46) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $399.00Supplier Page View Company Product Page
Catalog NumberNB100-1642
ImmunogenThis CD31/PECAM-1 Antibody (MEC 7.46) was developed against mouse endothelial cell line T-end
Concentration1 mg/ml
CloneMEC 7.46
Quantity0.1 ml (also 0.025 ml)
IsotypeIgG1
TargetCD31/PECAM-1
HostRat
FormatPBS
ReactivityMouse
ClonalityMonoclonal
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Flow (Cell Surface),Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
PurityProtein G purified
Research AreaAngiogenesis, Cancer, Cytoskeleton Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Myeloid Cell Markers, Signal Transduction, Stem Cell Markers
Molecular Weight82.5 kDa
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
NCBI Full Gene Nameplatelet/endothelial cell adhesion molecule 1, platelet and endothelial cell adhesion molecule 1
NCBI Gene AliasesPecam, CD31/EndoCAM, PECAM-1, CD31, PECA1, GPIIA', Cd31, endoCAM

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

What applications has the CD31/PECAM-1 antibody been used for in scientific research?

The CD31/PECAM-1 antibody has been shown to work in various applications including flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry (both frozen and paraffin-embedded tissues), immunoprecipitation, and fluorescence-activated cell sorting.

Can this antibody be used to study endothelial cell behavior in cancer models?

Yes, the CD31/PECAM-1 antibody has been utilized in studies examining endothelial cells in cancer models. For instance, it was used to assess neovascularization in breast cancer models, indicating its relevance in understanding tumor angiogenesis and the tumor microenvironment.

Is the CD31/PECAM-1 antibody effective for detecting endothelial cells in vivo?

The antibody has been effectively used in vivo, as demonstrated in studies where it was employed to stain tissue sections from glioma tumor models, confirming its utility in identifying endothelial cells within tumor vasculature.

What species does this antibody react with, and is it suitable for human studies?

The CD31/PECAM-1 antibody is reactive with both mouse and human tissues, making it suitable for studies involving both species, including translational research that may involve human samples.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoidsDevelopmental CellSeptember 22, 2025Yesl Jun, Kim-Vy Nguyen-Ngoc, Somesh Sai, R. Hugh F. Bender, Winnie Gong, Vira Kravets, Han Zhu, Christopher J. Hatch, Michael Schlichting, Roberto Gaetani, et al.anti-glucagon,Sigma-Aldrich,Cat# G2654 Sheep anti-CD31 (human),R&D Systems,Cat# AF806 Rat anti-CD31 (mouse),Novus,Cat# NB100–1642 Rabbit anti-laminin,Sigma-Aldrich,Cat# L9393 Mouse anti-laminin alpha 5,EMD Millipore,Cat# MAB1924 Goat

(2) Multimodal, PSMA-Targeted, PAMAM Dendrimer-Drug Conjugates for Treatment of Prostate Cancer: Preclinical EvaluationInt J NanomedicineMay 30, 2024Wojciech G Lesniak, Srikanth Boinapally, Gabriela Lofland, Zirui Jiang, Catherine A Foss, Babak Behman Azad, Anna Jablonska, Mauro A Garcia, Maria Brzezinski, Martin G PomperFluorescent anti-PSMA (Abcam, Cambridge, UK, ab66912) and anti-CD31-PE (Novus Biologicals, Centennial, CO, NB100-1642) were labeled as follows: 100 µg of anti-PSMA was dissolved in 100 µL of PBS, pH 7.4 in a microcentrifuge

(3) Multimodal, PSMA-Targeted, PAMAM Dendrimer-Drug Conjugates for Treatment of Prostate Cancer: Preclinical EvaluationInternational Journal of NanomedicineMay 30, 2024Wojciech G Lesniak, Srikanth Boinapally, Gabriela Lofland, Zirui Jiang, Catherine A Foss, Babak Behman Azad, Anna Jablonska, Mauro A Garcia, Maria Brzezinski, Martin G PomperFluorescent anti-PSMA (Abcam, Cambridge, UK, ab66912) and anti-CD31-PE (Novus Biologicals, Centennial, CO, NB100-1642) were labeled as follows: 100 µg of anti-PSMA was dissolved in 100 µL of PBS, pH 7.4 in a microcentrifuge

(4) Vascular endothelial profilin-1 drives a protumorigenic tumor microenvironment and tumor progression in renal cancerThe Journal of biological chemistryAugust 1, 2023Gau D, Daoud A, Allen A, Joy M, Sagan A, Lee S, Lucas PC, Duensing S, Boone D, Osmanbeyoglu HU, et al.secondary antibodies. The primary antibodies used were as follows: anti-CD31 antibody (77699, CST, 1:100 or NB1001642, Novus Biologicals, 1:100), anti-CD3 (78588, CST, 1:100), anti-CD8 antibody (98941, CST, 1:100), anti-F4/80

(5) Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrestLife science allianceAugust 1, 2023Acharya BR, Fang JS, Jeffery ED, Chavkin NW, Genet G, Vasavada H, Nelson EA, Sheynkman GM, Humphries MJ, Hirschi KK.(CIP1/WAF1),Abcam,Cat# ab218311 p57 KIP2,Cell Signaling Technology,Cat# 2557 c-MYC,Novus Biologicals,Cat# NB100-1642 Foxo3a,Cell Signaling Technology,Cat# 2497 Phospho-FoxO3a (Ser253),Cell Signaling Technology,Cat#

(6) FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2^-/- Mouse Model of Primary Sclerosing CholangitisHepatology communicationsJuly 1, 2022O'Brien A, Zhou T, White T, Medford A, Chen L, Kyritsi K, Wu N, Childs J, Stiles D, Ceci L, et al.

(7) Generation and identification of endothelial-specific Hrh2 knockout miceTransgenic researchJune 1, 2021Meng R, Cai WK, Xu WM, Feng Q, Wang P, Huang YH, Fan YX, Zhou T, Yang Q, Li ZR, et al.

(8) The effects of high fat diet, bone healing, and BMP-2 treatment on endothelial cell growth and functionBoneMay 1, 2021Bhatti FUR, Dadwal UC, Valuch CR, Tewari NP, Awosanya OD, de Andrade Staut C, Sun S, Mendenhall SK, Perugini AJ 3rd, Nagaraj RU, et al.

(9) Progenitor cell combination normalizes retinal vascular development in the oxygen-induced retinopathy (OIR) modelJCI insightNovember 1, 2019Li Calzi S, Shaw LC, Moldovan L, Shelley WC, Qi X, Racette L, Quigley JL, Fortmann SD, Boulton ME, Yoder MC, et al.

(10) Vascular Bed Molecular Profiling by Differential Systemic Decellularization In VivoArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2018Serra A, Gallart-Palau X, Park JE, Lim GGY, Lim KL, Ho HH, Tam JP, Sze SK.anti-ACTA2 (4 µg/mL, ab21027), from Abcam (Cambridge, UK) or rat monoclonal anti-PECAM1 (10 µg/mL, NB100-1642) from Novus Biologicals (Littleton, CO; please see the Major Resources Table in the online-only Data

(11) Mesenchymal stem cells expressing interleukin-18 inhibit breast cancer in a mouse modelOncology lettersMay 1, 2018Liu X, Hu J, Li Y, Cao W, Wang Y, Ma Z, Li F.incubating them with primary mouse anti-CD31/PECAM-1 monoclonal antibody (dilution, 1:150; cat. no. NB100-1642, Novus Biologicals, LLC, Littleton, CO, USA) and biotinylated goat anti-mouse IgG (dilution, 1:150;

(12) Host STING-dependent MDSC mobilization drives extrinsic radiation resistanceNature communicationsNovember 23, 2017Liang H, Deng L, Hou Y, Meng X, Huang X, Rao E, Zheng W, Mauceri H, Mack M, Xu M, et al.

(13) Spinal RacGAP α-Chimaerin Is Required to Establish the Midline Barrier for Proper Corticospinal Axon GuidanceThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 9, 2017Katori S, Noguchi-Katori Y, Itohara S, Iwasato T.

(14) Preexisting smooth muscle cells contribute to neointimal cell repopulation at an incidence varying widely among individual lesionsSurgeryFebruary 1, 2016Yang P, Hong MS, Fu C, Schmit BM, Su Y, Berceli SA, Jiang Z.β-galactosidase (ab9361, abcam, Cambridge, MA), rabbit anti CD31 (ab28364, abcam, Cambridge, MA), rat anti CD31 (NB100-1642, Novus, Littleton, CO), rabbit anti SMMHC (ab53219, abcam, Cambridge, MA), goat anti vimentin (sc-7557,

(15) Magnetic Resonance Q Mapping Reveals a Decrease in Microvessel Density in the arcAβ Mouse Model of Cerebral AmyloidosisFrontiers in aging neuroscienceJanuary 1, 2015Ielacqua GD, Schlegel F, Füchtemeier M, Xandry J, Rudin M, Klohs J.solution. Sections were incubated with the primary antibodies anti-murine CD31 (Rat, 1:250, catalog#NB100-1642; Novus Biological) and antiβ-amyloid (Mouse, 1:3000 Aβ1-16 specific, catalog#SIG 3920, Signet Covance)

(16) In vivo imaging of hypoxia-inducible factor regulation in a subcutaneous and orthotopic GL261 glioma tumor model using a reporter gene assayMolecular imagingJanuary 1, 2014Bürgi S, Seuwen A, Keist R, Vom Berg J, Grandjean J, Rudin M.according to standard immunofluorescence procedures. The following primary antibodies were used: CD31 (NB100-1642; Novus Europe, Cambridge, UK) 1/1,000; pimonidazole (fluorescein isothiocyanate– MAb1, Hypoxyprobe-1

(17) Oseltamivir phosphate monotherapy ablates tumor neovascularization, growth, and metastasis in mouse model of human triple-negative breast adenocarcinomaBreast cancer (Dove Medical Press)January 1, 2014Haxho F, Allison S, Alghamdi F, Brodhagen L, Kuta VE, Abdulkhalek S, Neufeld RJ, Szewczuk MR.

(18) Transcription factor Ets1 cooperates with estrogen receptor α to stimulate estradiol-dependent growth in breast cancer cells and tumorsPloS oneJanuary 1, 2013Kalet BT, Anglin SR, Handschy A, O'Donoghue LE, Halsey C, Chubb L, Korch C, Duval DL.

(19) VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of TiamMolecular biology of the cellApril 1, 2002Lampugnani MG, Zanetti A, Breviario F, Balconi G, Orsenigo F, Corada M, Spagnuolo R, Betson M, Braga V, Dejana E.

(20) Impact of the tumour bed effect on microenvironment, radiobiological hypoxia and the outcome of fractionated radiotherapy of human FaDu squamous-cell carcinoma growing in the nude mouseInternational journal of radiation biologyDecember 1, 2001Zips D, Eicheler W, Brüchner K, Jackisch T, Geyer P, Petersen C, van der Kogel AJ, Baumann M.

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