The Anti-CD123 [7G3], Rabbit IgG Antibody from MyBioSource.com is a Rabbit Monoclonal antibody to IL3RA. This antibody recognizes Human antigen. The Anti-CD123 [7G3], Rabbit IgG Antibody has been shown to work in the following applications: Block/Neutralize/Inhibit, Flow Cytometry, Immunoprecipitation, and Western Blot.
Description
This antagonist antibody binds IL-3Ralpha and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R alpha chain expressed by transfected cells and bound to primary cells expressing IL-3Ralpha. The expression of IL-3Ralpha chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Ralpha and betac dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcgammaRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514)