RPA2 [p Ser33] Antibody

Product Specs
ItemRPA2 [p Ser33] Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberNB100-544
Quantity100 ul
ApplicationsWestern Blot,Simple Western,Immunocytochemistry/ Immunofluorescence
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatTris-Citrate/Phosphate (pH 7.0 - 8.0)
PurityImmunogen affinity purified
ReactivityHuman,Mouse
HostRabbit
TargetRPA2
IsotypeIgG
Modificationsp Ser33
Molecular Weight32 kDa
Antibody TypePrimary Antibodies
NCBI Gene AliasesREPA2, RP-A p34, RPA32, RP-A p32
ImmunogenImmunogen was a phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33 using the numbering given in entry NP_002937.1 (GeneID 6118)
NCBI Full Gene Namereplication protein A2
ClonalityPolyclonal
Research AreaCancer, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, Nucleotide Excision Repair, Phospho Specific, Stem Cell Markers
StorageStore at 4C. Do not freeze

Specifications/Features

Available conjugates: Unconjugated

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Long-term exposure to the ethanol-derived metabolite acetaldehyde elevates structural genomic alterations but not base substitutionsCommunications BiologyJanuary 17, 2026Rita Lózsa, Bernadett Szikriszt, Eszter Németh, Zoltán Szeltner, Regina Martinek, Ádám Póti, Timóteus Feik, Sándor Kollarics, Bence G. Márkus, Nnennaya Kanu, et al.UK, #15310-1-AP), phospho-RPA32 (Ser33) antibody (Novus Biologicals, Bio-Techne, Minneapolis USA, #NB100-544), phospho-histone H2A.X (Ser139) antibody (Sigma-Aldrich, Missouri, USA, #ZMS05636), phospho-Chk1 (Ser317) antibody

(2) GSK3B directs DNA repair choice and determines tumor response to PARP1 inhibition independent of BRCA1Journal of Clinical InvestigationNovember 17, 2025Heba S. Allam, Scarlett Acklin-Wehnert, Ratan Sadhukhan, Mousumi Patra, Fen Xia9582), BRCA1 (Santa Cruz Biotechnology, SC-6954), Anti-RPA32/RPA2 (phospho S33) (Novus Biologicals, NB100–544), CtIP (Santa Cruz; sc-271339), and Rad51 (Abcam; ab133534). Secondary antibodies for Western blotting,

(3) GSK3B directs DNA repair choice and determines tumor response to PARP1 inhibition independent of BRCA1J Clin InvestNovember 17, 2025Heba S. Allam, Scarlett Acklin-Wehnert, Ratan Sadhukhan, Mousumi Patra, Fen Xia9582), BRCA1 (Santa Cruz Biotechnology, SC-6954), Anti-RPA32/RPA2 (phospho S33) (Novus Biologicals, NB100–544), CtIP (Santa Cruz; sc-271339), and Rad51 (Abcam; ab133534). Secondary antibodies for Western blotting,

(4) A parent-of-origin effect on embryonic telomere elongation determines telomere length inheritanceCurrent BiologyOctober 20, 2025Hyuk-Joon Jeon, Mia T. Levine, Michael A. Lampsonanti-DNA G-quadruplex structure (Millipore Sigma, MABE917), anti-RPA[phosphor Ser 33] (Novus Biologicals, NB100–544), anti-FLAG (Cell signaling, #2368). Secondary antibodies were Alexa Fluor 488-conjugated anti-mouse

(5) ARID1A Governs Genomic Stability and Proliferation in SCLC via c-MYC/PARP1 Suppression Driving Vulnerability to BET InhibitorsResearch (Wash D C)October 2, 2025Guozhen Cao, Liying Ma, Xueqin Dai, Peng Hou, Xinhuang Yao, Gongfeng Li, Jiahui Zhang, Ceshi Chen, Wenchu Lin(CST, #4858, 1:1,000), phospho-CHK1 (Ser317) (CST, #12302, 1:1,000), p-RPA2/32 (Ser4/Ser8) (Novus, NB100-544, 1:5,000), γ-H2AX (CST, #9718, 1:1,000), RAD51 (Abcam, ab133534, 1:1,000), RPA2/32 (Abcam, ab2175, 1:1,000),

(6) PARP1 auto-modification promotes faithful Okazaki fragment processing and limits replication fork speedMolecular cellOctober 2, 2025Elsborg JD, Munk SHN, Adelantado-Rubio A, Pék R, Fábian Z, Imburchia V, Zentout S, Hendriks IA, Buch-Larsen SC, Smith R, et al.

(7) ARID1A Governs Genomic Stability and Proliferation in SCLC via c-MYC/PARP1 Suppression Driving Vulnerability to BET InhibitorsResearch (Wash D C)October 2, 2025Guozhen Cao, Liying Ma, Xueqin Dai, Peng Hou, Xinhuang Yao, Gongfeng Li, Jiahui Zhang, Ceshi Chen, Wenchu Lin(CST, #4858, 1:1,000), phospho-CHK1 (Ser317) (CST, #12302, 1:1,000), p-RPA2/32 (Ser4/Ser8) (Novus, NB100-544, 1:5,000), γ-H2AX (CST, #9718, 1:1,000), RAD51 (Abcam, ab133534, 1:1,000), RPA2/32 (Abcam, ab2175, 1:1,000),

(8) Cyclin D1 overexpression induces replication stress and microhomology-mediated end-joining dependence in mantle cell lymphomaJournal of Clinical InvestigationSeptember 2, 2025Jithma P. Abeykoon, Shuhei Asada, Guangli Zhu, Yuna Hirohashi, Lisa Moreau, Divya R Iyer, Sirisha Mukkavalli, Kalindi Parmar, Gabriella Zambrano, Lige Jiang, et al.MilliporeSigma; catalog 05-636-MI; clone JBW301), anti–p-RPA2 S33 (rabbit polyclonal; Novus Biologicals; catalog NB100544), and anti-V5 (rabbit monoclonal; CST; catalog 13202; clone D3H8Q). Comet assay. The alkaline comet assays

(9) Cyclin D1 overexpression induces replication stress and microhomology-mediated end-joining dependence in mantle cell lymphomaJ Clin InvestJuly 3, 2025Jithma P. Abeykoon, Shuhei Asada, Guangli Zhu, Yuna Hirohashi, Lisa Moreau, Divya Iyer, Sirisha Mukkavalli, Kalindi Parmar, Gabriella Zambrano, Lige Jiang, et al.MilliporeSigma; catalog 05-636-MI; clone JBW301), anti–p-RPA2 S33 (rabbit polyclonal; Novus Biologicals; catalog NB100544), and anti-V5 (rabbit monoclonal; CST; catalog 13202; clone D3H8Q). Comet assay. The alkaline comet assays

(10) CRAMP1-dependent histone H1 biogenesis is essential for topoisomerase II inhibitor toleranceMolecular cellJuly 3, 2025Ingham A, de Vega IA, Morlot L, Gittens W, Hendriks IA, Kakulidis ES, Freire R, Davey NE, Duxin JP, Lund Nielsen M, et al.Scientific,"(Thermo Fisher Scientific Cat# A300-767A, RRID:AB_669740)" pRPA2 (Ser33),Novus,"(Novus Cat# NB100-544, RRID:AB_10002227)" RPA2,Santa Cruz Biotechnology,"(Santa Cruz Biotechnology Cat# sc-56770, RRID:AB_785534)" ß-Actin,Sigma-Aldrich,"(Sigma-Aldrich

(11) The class II myosin MYH4 safeguards genome integrity and suppresses tumor progressionJ Clin InvestJune 2, 2025Jayashree Thatte, Ana Moisés da Silva, Judit Börcsök, Thorkell Gudjónsson, Jan Benada, Xin Li, Muthiah Bose, Hanneke van der Gulden, Ji-Ying Song, Renée Menezes, et al.Cell Signaling Technology, 2577) or mouse anti-γH2AX (1:1000; Millipore, MABE285), anti–p-RPA (S33) (1:500; Novus, NB100-544), mouse anti-MCM2/BM28 (1:500; BD Biosciences, 610700), rabbit anti–p-MCM2 (S40/S41) (1:500; Bethyl Laboratories

(12) The class II myosin MYH4 safeguards genome integrity and suppresses tumor progressionJ Clin InvestJune 2, 2025Jayashree Thatte, Ana Moisés da Silva, Judit Börcsök, Thorkell Gudjónsson, Jan Benada, Xin Li, Muthiah Bose, Hanneke van der Gulden, Ji-Ying Song, Renée Menezes, et al.Cell Signaling Technology, 2577) or mouse anti-γH2AX (1:1000; Millipore, MABE285), anti–p-RPA (S33) (1:500; Novus, NB100-544), mouse anti-MCM2/BM28 (1:500; BD Biosciences, 610700), rabbit anti–p-MCM2 (S40/S41) (1:500; Bethyl Laboratories

(13) Haplotype-resolved de novo assembly revealed unique characteristics of alternative lengthening of telomeres in mouse embryonic stem cellsNucleic acids researchNovember 11, 2024Lee H, Niida H, Sung S, Lee J.(Biolegend #613401, 1/1000 dilution), 53BP1 (NOVUS, NB100-304, 1/1000 dilution), RPA2 [p Ser33] (NOVUS, NB100-544, 1/1000 dilution). PNA probe: TelG-Cy3 ((TTAGGG)*4-Cy3), TelC-FITC ((CCCTAA)*4-FITC) Chromosome orientation-FISH

(14) Enhancing transcription-replication conflict targets ecDNA-positive cancersNatureNovember 1, 2024Tang J, Weiser NE, Wang G, Chowdhry S, Curtis EJ, Zhao Y, Wong IT, Marinov GK, Li R, Hanoian P, et al.

(15) Discovery of a 6-Aminobenzo[b]thiophene 1,1-Dioxide Derivative (K2071) with a Signal Transducer and Activator of Transcription 3 Inhibitory, Antimitotic, and Senotherapeutic ActivitiesACS pharmacology & translational scienceSeptember 13, 2024Oleksak P, Rysanek D, Vancurova M, Vasicova P, Urbancokova A, Novak J, Maurencova D, Kashmel P, Houserova J, Mikyskova R, et al.

(16) A molecular glue RBM39-degrader induces synthetic lethality in cancer cells with homologous recombination repair deficiencynpj Precision OncologyMay 24, 2024Shinji Kohsaka, Shigehiro Yagishita, Yukina Shirai, Yusuke Matsuno, Toshihide Ueno, Shinya Kojima, Hiroshi Ikeuchi, Masachika Ikegami, Rina Kitada, Ken-ichi Yoshioka, et al.Diego, CA, USA), rabbit anti-Rad51 (#ab133534, Abcam, Cambridge, UK), rabbit anti-phospho RPA2 (Ser33) (#NB100-544, Novus Biologicals, Littleton, CO, USA), or rabbit anti-53BP1 (#PC712, Merck) in blocking buffer for

(17) The SPATA5-SPATA5L1 ATPase complex directs replisome proteostasis to ensure genome integrityCellApril 25, 2024Krishnamoorthy V, Foglizzo M, Dilley RL, Wu A, Datta A, Dutta P, Campbell LJ, Degtjarik O, Musgrove LJ, Calabrese AN, et al.

(18) Human papillomavirus E7 protein induces homologous recombination defects and PARPi sensitivityJournal of cancer research and clinical oncologyJanuary 23, 2024He S, Wang A, Wang J, Tang Z, Wang X, Wang D, Chen J, Liu C, Zhao M, Chen H, et al.for immunoblotting included: anti-NBS1 phosphor-Serine 343 (Abcam, ab109453), anti-RPA32 phosphor-Serine 33 (Novus, NB100-544), anti-CHK2 phosphor-Threonine 68 (CST, 2348), anti-CHK1 phosphor-Serine 345 (CST, 2348S), anti-p53

(19) SIRT1 regulates DNA damage signaling through the PP4 phosphatase complexNucleic acids researchJuly 21, 2023Rasti G, Becker M, Vazquez BN, Espinosa-Alcantud M, Fernández-Duran I, Gámez-García A, Ianni A, Gonzalez J, Bosch-Presegué L, Marazuela-Duque A, et al.anti-phospho-histone H2A.X (Ser139) (abcam ab 2893 and Merck Millipore, JBW301), phospho-RPA2 (Ser33) (Novus, NB100-544 and Bethyl, A300-246A), phospho-RPA2 (Ser4/8) (Sigma-Aldrich, PLA0071) and Fibrillarin (Santa Cruz Biotechnology,

(20) The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancerCommunications biologyJune 22, 2023Yeo XH, Sundararajan V, Wu Z, Phua ZJC, Ho YY, Peh KLE, Chiu YC, Tan TZ, Kappei D, Ho YS, et al.from Cell signalling; anti-SAM68 (07-415) and anti-γH2AX (3BW301) from Millipore; antiRPA2 [p Ser33] (NB100-544) from Novus Biologicals; anti-SAM68 (ab76471), anti-SREBP2 (30682), anti-HMGCR (242315) from Abcam;

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