Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) NB7185 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemDonkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)
CompanyNovus Biologicals, a Bio-Techne Brand
Price $219.00Supplier Page View Company Product Page
Catalog NumberNB7185
ImmunogenThis Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was developed against rabbit IgG-heavy and light chain
Concentration0.5 mg/ml
FormatPhosphate Buffered Saline (PBS) containing 0.2% BSA
ReactivityRabbit
Quantity1 ml
HostDonkey
ApplicationsWestern Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Conjugate/TagHRP
TargetIgG (H+L)
IsotypeIgG
ClonalityPolyclonal
PurityImmunogen affinity purified
Antibody TypeSecondary Antibodies
Research AreaAdaptive Immunity, Immunology
StorageStore at 4C. Do not freeze

Specifications/Features

Available conjugates: Available conjugates: HRP

Specificity: By immunoelectrophoresis and ELISA this Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

Sizes Available: 1 ml

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) A new mouse model of Charcot-Marie-Tooth 2J neuropathy replicates human axonopathy and suggest alteration in axo-glia communicationPLoS geneticsNovember 1, 2022Shackleford G, Marziali LN, Sasaki Y, Claessens A, Ferri C, Weinstock NI, Rossor AM, Silvestri NJ, Wilson ER, Hurley E, et al.peroxidase-conjugated secondary antibodies: 1:20,000 donkey anti-rabbit IgG(H+L) polyclonal (Novus Cat# NB7185) and peroxidase-AffiniPure donkey anti-chicken IgY antibody (Jackson ImmunoResearch Cat# 703–035–155).

(2) IFNγ induces JAK1/STAT1/p65 NFκB-dependent interleukin-8 expression in ovarian cancer cells, resulting in their increased migrationThe international journal of biochemistry & cell biologyDecember 1, 2021Padmanabhan S, Gaire B, Zou Y, Uddin MM, DeLeon D, Vancurova I.in TBSTM. After washing, the membranes were incubated with HRP-labeled secondary anti-rabbit (Novus NB7185) or anti-mouse (Novus NB7570) antibodies (dilution 1:5000) and the labeled proteins were detected using

(3) "Deletion of Calcineurin in Schwann Cells Does Not Affect Developmental Myelination, But Reduces Autophagy and Delays Myelin Clearance after Peripheral Nerve Injury"The Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 1, 0001"Reed CB, Frick LR, Weaver A, Sidoli M, Schlant E, Feltri ML, Wrabetz L."donkey anti-chicken 800W 1:10,000 (LI-COR, 926-32218), donkey antirabbit 1:10,000 (Novus Biologicals, NB7185), goat anti-rabbit IgG 800CW 1:10,000 (LI-COR, 92632211), goat anti-rabbit IgG 670CW 1:10,000 (LI-COR,

(4) Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-CorrectionNeuronJanuary 1, 0001"Weinstock NI, Shin D, Dhimal N, Hong X, Irons EE, Silvestri NJ, Reed CB, Nguyen D, Sampson O, Cheng YC, et al."

(5) Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cellsOncogeneJanuary 1, 0001"Dovmark TH, Saccomano M, Hulikova A, Alves F, Swietach P."Bratislava, Slovakia). Membranes were then incubated with HRP-conjungated secondary antibodies (Novus NB7256, NB7185 or NB120-7061) and developed using ECL (Thermo). Blots were repeated three times on independent lysates. Solutions

(6) "Immune checkpoint protein PD-L1 promotes transcription of angiogenic and oncogenic proteins IL-8, Bcl3, and STAT1 in ovarian cancer cells"The Journal of biological chemistryJanuary 1, 0001"Reddy SU, Sham R, Smith K, Gaire B, Vancura A, Vancurova I."with horseradish peroxidase–conjugated anti-rabbit immunoglobulin G (Novus Biologicals, catalog no.: NB7185; 1:5000 dilution), and the labeled proteins were detected using ECL Western Detection Reagent (Amersham)

(7) Saponin attenuates diesel exhaust particle (DEP)-induced MUC5AC expression and pro-inflammatory cytokine upregulation via TLR4/TRIF/NF-κB signaling pathway in airway epithelium and ovalbumin (OVA)-sensitized miceJournal of ginseng researchJanuary 1, 0001"Jo S, Na HG, Choi YS, Bae CH, Song SY, Kim YD."

(8) "IFNγ-induced PD-L1 expression in ovarian cancer cells is regulated by JAK1, STAT1 and IRF1 signaling"Cellular signallingJanuary 1, 0001"Padmanabhan S, Gaire B, Zou Y, Uddin MM, Vancurova I."in TBSTM. After washing, the membranes were incubated with HRP-labeled secondary anti-rabbit (Novus NB7185) or anti-mouse (Novus NB7570) antibodies (dilution 1:5000) and the labeled proteins were detected using

(9) UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytesExperimental & molecular medicineJanuary 1, 0001"Chae M, Son ED, Bae IH, Cho EG, Kim HJ, Jung JY."

(10) Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinomaCell death & diseaseJanuary 1, 0001"Akuma M, Kim M, Zhu C, Wiljer E, Gaudreau-Lapierre A, Patterson LD, Egevad L, Tanguay S, Trinkle-Mulcahy L, Stanford WL, et al."from Aviva systems biology. Peroxidase-conjugated sheep anti-mouse (NB1206808) and donkey anti-rabbit (NB7185) antibodies were purchased from Novus Biologicals. Antibodies used for co-immunoprecipitation include

(11) Loss of prohibitin 2 in Schwann cells dysregulates key transcription factors controlling developmental myelinationGliaJanuary 1, 0001"Wilson ER, Nunes GD, Shen S, Moore S, Gawron J, Maxwell J, Syed U, Hurley E, Lanka M, Qu J, et al."washed three times in 1x TBS-T and incubated with HRP-conjugated secondary antibody (Novus Biologicals NB7185) for 1 h at room temperature. Following three further washes in TBS-T, membranes were developed using

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Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (11)

Reviews

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) from Novus Biologicals, a Bio-Techne Brand

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