Granzyme B Antibody NB100-684 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGranzyme B Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $699.00Supplier Page View Company Product Page
Catalog NumberNB100-684
Conjugate/TagUnconjugated
FormatPBS (pH 7.4), 0.2% BSA, Tween-20
HostRabbit
ReactivityHuman,Mouse
ApplicationsIF/IHC,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry/ Immunofluorescence
Quantity0.5 ml (also 0.1 ml)
TargetGranzyme B
ImmunogenSynthetic peptide: EIIGGHEAKPHSRPYMAYL, corresponding to amino acids 20-38 of Human Granzyme B (N-terminus)
ClonalityPolyclonal
PurityAffinity purified
IsotypeIgG
Antibody TypePrimary Antibodies
Research AreaApoptosis
StorageStore at 4C. Do not freeze
NCBI Full Gene Namegranzyme B
NCBI Gene AliasesC11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: This reacts with a 32 kDa protein.

Sizes Available: 0.5 ml (also 0.1 ml)

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Questions and Answers

Can this Granzyme B antibody be used effectively in flow cytometry applications?

Yes, the Granzyme B antibody has been shown to work in flow cytometry, as indicated by its use in a study where it was employed to analyze cytotoxic T cell activity and granzyme B production in a lymphoma coculture system.

What species does this antibody react with, and is it suitable for human studies?

This antibody reacts with both mouse and human species, making it suitable for studies involving human samples, particularly in the context of apoptosis research.

How has this antibody been utilized in the context of immunotherapy research?

The Granzyme B antibody has been cited in research demonstrating its role in enhancing the cytotoxic activity of CD8+ T cells and natural killer cells, particularly in studies investigating the synergistic effects of high-dose ascorbic acid and anti-PD1 therapy in lymphoma models.

What are the recommended dilution and conditions for using this antibody in immunofluorescence?

For immunofluorescence applications, the Granzyme B antibody is recommended to be used at a dilution of 1:200, as noted in the literature, which supports its effective visualization in cellular assays.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Unbiased niche labeling maps immune-excluded niche in bone metastasisCellMay 28, 2026Zhan Xu, Fengshuo Liu, Yunfeng Ding, Tianhong Pan, Yi-Hsuan Wu, Yujiao Han, Jun Liu, Igor L. Bado, Weijie Zhang, Ling Wu, et al.,Novus Biologicals,Cat#NB100-1614; RRID:AB_10001164 "rabbit anti-Granzyme B, 1:200",Novus Biologicals,Cat#NB100-684; RRID:AB_10001094 "rat anti-mouse CD8a, 1:200",Thermo Fisher Scientific,Cat#14-0195-82; RRID:AB_2637159 Mouse

(2) Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of MacrophagesCell reportsJanuary 1, 0001"Na YR, Kwon JW, Kim DY, Chung H, Song J, Jung D, Quan H, Kim D, Kim JS, Ju YW, et al."

(3) Bruton Tyrosine Kinase-Dependent Immune Cell Cross-talk Drives Pancreas CancerCancer discoveryJanuary 1, 0001"Gunderson AJ, Kaneda MM, Tsujikawa T, Nguyen AV, Affara NI, Ruffell B, Gorjestani S, Liudahl SM, Truitt M, Olson P, et al."

(4) "Efficient ADCC killing of meningioma by avelumab and a high-affinity natural killer cell line, haNK"JCI insightJanuary 1, 0001"Giles AJ, Hao S, Padget M, Song H, Zhang W, Lynes J, Sanchez V, Liu Y, Jung J, Cao X, et al."serial sections were prepared from FFPE xenograft tissues. Immunohistochemistry staining for GZMB (NB100-684, Novus Biologicals) and cleaved caspase-3 (Asp175, Cell Signaling Technology) were performed by HistoServ,

(5) Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8⁺ T cell exclusionJournal of experimental & clinical cancer research : CRJanuary 1, 0001"Fang W, Zhou T, Shi H, Yao M, Zhang D, Qian H, Zeng Q, Wang Y, Jin F, Chai C, et al."(Cell Signaling Technology, 25229S, 1:200), CD8 (Cell Signaling Technology, 98941S, 1:200), Granzyme B (NOVUS, NB100–684, 1:200), and PD-1 (Abcam, ab214421, 1:200). IHC stainings were examined with microscopy. Immunofluorescence Slides

(6) High-dose ascorbic acid synergizes with anti-PD1 in a lymphoma mouse modelProceedings of the National Academy of Sciences of the United States of AmericaJanuary 1, 0001"Luchtel RA, Bhagat T, Pradhan K, Jacobs WR Jr, Levine M, Verma A, Shenoy N."no.,Dilution,Host,Channel Cd11c,Biorbyt/orb13554,1:100,Rabbit,Cy5 F4/80,Abcam/ab111101,1:400,Rabbit,Cy5 Granzyme B,Novus Biologicals/NB100-684,1:200,Rabbit,Cy5 PD-L1,Cell Signaling/13684,1:250,Rabbit,Cy5 CD8,Thermo Fisher/14-0808-82,1:200,Rat,Cy5/TRITC

(7) Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathologyNature immunologyJanuary 1, 0001"Fujiwara H, Seike K, Brooks MD, Mathew AV, Kovalenko I, Pal A, Lee HJ, Peltier D, Kim S, Liu C, et al."

(8) Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to ChemotherapyCancer cellJanuary 1, 0001"Medler TR, Murugan D, Horton W, Kumar S, Cotechini T, Forsyth AM, Leyshock P, Leitenberger JJ, Kulesz-Martin M, Margolin AA, et al."Pharmingen; 1:500), F4/80 (Cl:A3–1; Serotech; 1:500), C5aR1 (10/92; Abcam; 1:500), or Granzyme B (#NB100–684; Novus Biologicals; 1:200). Tissue sections were then incubated with the corresponding biotinylated

(9) IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusionThe Journal of experimental medicineJanuary 1, 0001"Chen X, Zhao J, Herjan T, Hong L, Liao Y, Liu C, Vasu K, Wang H, Thompson A, Fox PL, et al."(A00421-2, 1:150; Boster); anti-human IL-17A for staining (AF-317, 1:50; R&D); anti-GzmB for staining (NB100-684, validated in mouse, 1:150; Novus); anti-CD45 for staining (AF114, 1:150; R&D); and anti-mouse Gr-1

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