Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7186] - An inhibitor of EZH2 reactivates miR-125b-1 in MCF7 breast cancer cells. To determine the dose of GSK126 that reduces H3K27me3 levels, we treated the breast cell lines with different GSK126 concentrations and performed Western blot analysis.