Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] NB7160 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGoat anti-Rabbit IgG (H+L) Secondary Antibody [HRP]
CompanyNovus Biologicals, a Bio-Techne Brand
Price $249.00Supplier Page View Company Product Page
Catalog NumberNB7160
ImmunogenThis Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] was developed against rabbit IgG-heavy and light chain
Concentration1.0 mg/ml
Conjugate/TagHRP
ApplicationsWestern Blot, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
ReactivityRabbit
Quantity1 ml
FormatPBS, 0.2% BSA
HostGoat
TargetIgG (H+L)
IsotypeIgG
ClonalityPolyclonal
PurityImmunogen affinity purified
Antibody TypeSecondary Antibodies
Research AreaAdaptive Immunity, Immunology
StorageStore at 4C in the dark

Specifications/Features

Available conjugates: Available conjugates: DyLight 594, DyLight 550, FITC, DyLight 350, HRP, DyLight 755, Allophycocyanin, DyLight 680, PerCP, DyLight 405, DyLight 488, DyLight 650

Specificity: By immunoelectrophoresis and ELISA this Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Sizes Available: 1 ml

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Questions and Answers

Can this secondary antibody be used for Western blotting in my experiments?

Yes, the Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] has been successfully utilized in Western blotting applications, as evidenced by its use in multiple studies, including those by Bigot et al. (2016) and El Assar et al. (2022), where it was employed at a dilution of 1:5,000.

What is the specificity of this antibody, and how does it perform in immunofluorescence?

This antibody specifically reacts with rabbit IgG and light chains common to other rabbit immunoglobulins, making it suitable for applications such as immunofluorescence. It has been cited in studies, including those by Gunasekar et al. (2019) and Lau et al. (2024), where it was used to detect target proteins in various cell types.

Is this antibody effective for immunohistochemistry applications?

Yes, the Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] has been shown to work effectively in immunohistochemistry applications, as referenced in studies like Rudolf et al. (2024), where it was used to visualize specific proteins in tissue sections.

What is the recommended storage condition for this antibody to maintain its stability?

The antibody should be stored at 4°C in the dark to maintain its stability and effectiveness for experimental use.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Molecular and functional characterization of telomeric repeat-containing RNAs in Chinese hamster ovary cellsNucleic Acids ResJuly 27, 2026Beatriz Domingues-Silva, André F Seixas, Claus M Azzalinantibodies were HRP-conjugated goat anti-mouse and anti-rabbit IgGs (Novus Biologicals, NB7539 and NB7160, 1:3000). DNA fluorescence in situ hybridization (FISH) on metaphase spreads Cells were treated with

(2) CD8+ T cells cross-restricted by HLA-B*57 and HLA-E*01 recognize HIV Gag with different functional profilesJCI InsightFebruary 9, 2026Kevin J. Maroney, Michael A. Rose, Allisa K. Oman, Abha Chopra, Hua-Shiuan Hsieh, Zerufael Derza, Rachel Waterworth, Mark A. Brockman, Spyros A. Kalams, Anju Bansal, et al.4°C, then, after another round of washing, 50 μL secondary antibody (1:500; anti-rabbit–HRP, catalog NB7160, Novus Biologicals) for 15 minutes at room temperature in the dark. Finally, the plate was washed again,

(3) Methionine Restriction Reduces Lung Cancer Progression and Increases Chemotherapy ResponsebioRxivJanuary 16, 2026Kassandra J. Naughton, Xiulong Song, Avery R. Childress, Erika M. Skaggs, Aria L. Byrd, Christian M. Gosser, Dave-Preston Esoe, Tanner J. DuCote, Daniel R. Plaugher, Alexsandr Lukyanchuk, et al.incubated at RT for 1 hour with secondary antibody (mouse Novus CAT#: NB7539, and rabbit Novus CAT#: NB7160) diluted 1:10,000 in TBST. They were then washed for 20 minutes 3x in TBST at RT. H3 strips were subject

(4) Transcription Activation of DEPDC1B Upon EBF1 Loss Contributes to Cell Cycle Progression and Epithelial-Mesenchymal Transition in Colon AdenocarcinomaBiochemical GeneticsDecember 16, 2025Yang Li, Xin Shi, Baoyong Ling, Xiangyu Su, Jing Zhangab6046, Abcam). Afterward, the membranes were incubated for 1 h at 37℃ with secondary antibody (1:5000, NB7160, Novus Biologicals, LLC, Littleton, CO, USA). The signals were developed with the enhanced chemiluminescence

(5) Oral administration of the probiotic strain Lactobacillus helveticus BGRA43 reduces high-fat diet–induced hepatic steatosis in mice and restores gut microbiota compositionFrontiers in PharmacologyNovember 4, 2025Ana Teofilović, Miloš Vratarić, Biljana Bursać, Ljupka Gligorovska, Danijela Vojnović Milutinović, Nemanja Stanisavljević, Ivana Strahinić, Danijela Mišić, Filip Nikolić, Bojan Pavlović, et al.the appropriate horseradish peroxidase-conjugated secondary antibodies: Anti-Rabbit IgG (1:20,000, NB7160, Novus Biologicals) and Anti-Mouse IgG (1:30,000, ab97046, Abcam). Anti-β-actin (1:10,000, ab8227, Abcam)

(6) Gut microbiota composition combined with reduced intestinal fatty acid uptake prevents hepatic steatosis in obesity-resistant mice fed a high-fat dietScientific reportsOctober 23, 2025Vratarić M, Teofilović A, Vojnović Milutinović D, Veličković N, Đmura G, Djordjevic A.the corresponding horseradish peroxidase-conjugated secondary antibodies (Anti-Rabbit IgG: 1:20000, NB7160, Novus Biologicals; Anti-Mouse IgG: 1:30000, ab97046, Abcam). Anti-β-actin (1:10000, ab8227, Abcam)

(7) Programmed cell revival from imminent cell death enhances tissue repair and regenerationThe EMBO JournalAugust 21, 2025Kollori Dhar, Kautilya Kumar Jena, Subhash Mehto, Rinku Sahu, Krushna C Murmu, Atharva Anand Mahajan, Sibaram Behera, Ravi Kiran Putchala, Reuben Jacob Mathew, Ramyasingh Bal, et al.,Novus Biologicals,Cat#NB7539 Goat anti-Rabbit IgG (H + L) Secondary Antibody [HRP],Novus Biologicals,Cat#NB7160 p-Histone H2A.X (Ser 139) (Mouse-mAb),Santa Cruz,Cat#sc-517348; RRID:AB_2783871 p21 Waf1/Cip1 (12D1) (Rabbit-mAb

(8) GMP‐compliant manufacturing of biologically active cell‐derived vesicles produced by extrusion technologyJournal of Extracellular BiologyDecember 25, 2022Hui‐Chong Lau, Dong Woo Han, Jinhee Park, Edwine Lehner, Carina Kals, Claudia Arzt, Elisabeth Bayer, Daniela Auer, Tanja Schally, Eva Grasmann, et al.anti‐mouse (Novus Biological, #NB7539) for CD63, CD81 and CD9; goat anti‐rabbit (Novus Biological, #NB7160) for CD29, LAMP1, flotillin‐1, lamin B1, calnexin, citrate synthase and GM130; or anti‐rabbit IgGs,

(9) Adenosine receptor expression in the adult zebrafish retinaPurinergic signallingJanuary 1, 0001"Grillo SL, McDevitt DS, Voas MG, Khan AS, Grillo MA, Stella SL Jr."anti-β-actin (cat. no. A5316-100UL, Sigma) was used for the loading control. Goat anti-rabbit (cat. no. NB7160, Novus Biologicals, Centennial, CO) and goat antimouse (cat. no. ab205719, AbCam, Cambridge, MA) secondary

(10) Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometryScientific reportsJanuary 1, 0001"Lau HC, Passalacqua I, Jung JH, Kwon Y, Zocco D, Park SS, Oh SW."anti-mouse (Novus Biological, #NB7539) for CD63, CD81, CD9, and CD147; goat anti-rabbit (Novus Biological, #NB7160) for ITGB1, LAMP1, NCSTN, ATP1B3, and PTGFRN, at 1:2,500 ratio for 1 h. Lastly, the membrane was washed

(11) "Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice"Cell and tissue researchJanuary 1, 0001"Ganburged G, Suda N, Saito M, Yamazaki Y, Isokawa K, Moriyama K."

(12) Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identificationInternational journal of systematic and evolutionary microbiologyJanuary 1, 0001"Roesler U, Scholz H, Hensel A."

(13) Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytesJournal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)January 1, 0001"Lu Y, Kim C, Collins JF."

(14) Memantine/Aripiprazole Combination Alleviates Cognitive Dysfunction in Valproic Acid Rat Model of Autism: Hippocampal CREB/BDNF Signaling and Glutamate HomeostasisNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsJanuary 1, 0001"Zohny SM, Habib MZ, Mohamad MI, Elayat WM, Elhossiny RM, El-Salam MFA, Hassan GAM, Aboul-Fotouh S."times for 5 min with TBST. Incubation was done in Goat anti-Rabbit IgG Secondary Antibody [HRP] (cat# NB7160; Novus Biologicals, Centennial, CO, USA) solution against the blotted target protein for 1 h at room

(15) The effects of intermittent hypoxia on hepatic expression of fatty acid translocase CD36 in lean and diet-induced obese miceBiomedical journalJanuary 1, 0001"Ji Y, Liang Y, Chu PH, Ge M, Yeung SC, Ip MSM, Mak JCW."peroxidase (HRP)-conjugated goat-anti-rabbit or HRP-conjugated goat-anti-mouse IgG secondary antibodies (Cat#NB7160, NB7544, Novus Biologicals, Littleton, CO, USA, 1:5000), and visualized by the ChemiDoc MP Imaging System

(16) "Xanthenone, ACE2 activator, counteracted gentamicin-induced nephrotoxicity in rats: Impact on oxidative stress and ACE2/Ang-(1-7) signaling"Life sciencesJanuary 1, 0001"Abdel-Fattah MM, Elgendy ANAM, Mohamed WR."

(17) Functional and histological evaluation of bone marrow stem cell-derived exosomes therapy on the submandibular salivary gland of diabetic Albino rats through TGFβ/ Smad3 signaling pathwayHeliyonJanuary 1, 0001"AbuBakr N, Haggag T, Sabry D, Salem ZA."

(18) Dexamethasone represses signaling through the mammalian target of rapamycin in muscle cells by enhancing expression of REDD1The Journal of biological chemistryJanuary 1, 0001"Wang H, Kubica N, Ellisen LW, Jefferson LS, Kimball SR."

(19) Insufficient autophagy promotes bronchial epithelial cell senescence in chronic obstructive pulmonary diseaseOncoimmunologyJanuary 1, 0001"Fujii S, Hara H, Araya J, Takasaka N, Kojima J, Ito S, Minagawa S, Yumino Y, Ishikawa T, Numata T, et al."

(20) Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male ratsPloS oneJanuary 1, 0001"Ogura Y, Kakehashi C, Yoshihara T, Kurosaka M, Kakigi R, Higashida K, Fujiwara SE, Akema T, Funabashi T."

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