RASEF Antibody MA5-44711 from Thermo Fisher Scientific

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RASEF Antibody

Immunohistochemistry analysis of RAB45 in paraffin-embedded rat large intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:100 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Western blot analysis of RAB45 in different lysates (10 µg/Lane). Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in RAB45 Monoclonal antibody (Product # MA5-44711) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Lane 1: K562 cell lysate; Lane 2: A431 cell lysate; Lane 3: RAW264.7 cell lysate; Lane 4: NIH/3T3 cell lysate. Predicted band size: 83 kDa. Observed band size: 85 kDa.

Supplier Page from
Thermo Fisher Scientific for
RASEF Antibody

Thermo Fisher Scientific's RASEF Antibody is a Rabbit monoclonal antibody. The RASEF Antibody was generated using RASEF as the antigen. It reacts with Homo Sapiens, Human, Mouse, and Rat. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, and Western Blot.

RASEF Antibody

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RASEF Antibody from Thermo Fisher Scientific

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RASEF Antibody from Thermo Fisher Scientific

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