Immunocytochemistry analysis of RBM3 in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in RBM3 Monoclonal antibody (Product # MA5-44679) using a dilution of 1:50 in 2% negative goat serum overnight at 4 ℃ followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.