Western blot analysis of HSP60 in tissue or whole cell lysates. Samples were incubated in HSP60 monoclonal antibody (Product # MA5-47385) using a dilution of 1:10,000 (green). [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. Strong single band corresponds to HSP60 protein with apparent SDS-PAGE molecular weight of 60 kDa.