Immunocytochemistry-Immunofluorescence analysis of Rabbit anti-Mouse IgG, Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 (Product # A56576, 1 µg/mL) was performed on fixed and permeabilized HeLa cells stained with Mouse beta Tubulin Monoclonal Antibody (2 28 33) (Product # 32-2600, 1:500). Panel a) shows representative cells that were stained for detection and localization of beta Tubulin protein, Panel b) is stained for nuclei (blue) using NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) (Product # R37606). Panel c) represents HSPA9 protein stained using Goat HSPA9 Polyclonal Antibody and appropriate secondary antibody conjugated to Alexa Fluor™ Plus 488. Panel d) is a composite image of Panels a, b and c clearly demonstrating specific detection of mouse beta Tubulin Monoclonal Antibody by Rabbit anti-Mouse IgG, Recombinant Secondary Antibody, Alexa Fluor™ Plus 647. Panel e) represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.