Western blot analysis of extracts of 293T cells, using Phospho-Ezrin-Y353 pAb (TA376054) at 1:1000 dilution or Ezrin antibody (A0703).293T cells were treated by ATP(5 mM) at 30‚ÑÉ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.