Western blot analysis of extracts of C6 cells, using Phospho-NMDAR1-S890 antibody (TA376782) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.