Western blot analysis of extracts of HeLa cells, using Phospho-NMDAR1-S896/S897 antibody (TA376783) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.