Western blot analysis of extracts of various cell lines, using Phospho-DDR1-Y792 antibody (TA375378) at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30‚ÑÉ for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30‚ÑÉ for 1 hour. C6 cells were treated by ATP(5 mM) at 30‚ÑÉ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.