Western blot analysis of extracts of Mouse brain, using Phospho-Tau-Thr231 antibody (TA378335) at 1:1000 dilution.Mouse brain were treated by CIP (20ul CIP for each 400ul cell lysate) at 37‚ÑÉ for 1 hour. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.