Western blot analysis of extracts of HeLa cells, using Phospho-MCM2-S139 antibody (TA378401) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour.HeLa cells were treated by Serum-starvation overnight at 37‚ÑÉ.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.