Western blot analysis of extracts of various cell lines, using SMPD1 / ASM antibody (TA381793) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Immunofluorescence analysis of NIH-3T3 cells using SMPD1 / ASM Polyclonal Antibody (TA381793) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.