Western blot analysis of extracts of various cell lines, using LC3B antibody (TA378232) at 1:500 dilution.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Immunofluorescence analysis of NIH-3T3 treated by chloroquine and NIH-3T3 cells using [KO Validated] LC3B Rabbit pAb (TA378232) at dilution of 100 (40x lens).NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.