Western blot analysis of extracts of HeLa cells, using Phospho-E-Cadherin-S838/S840 antibody (TA374522) at 1:1000 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.