Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKC mu-S744/748 pAb (TA380374) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 5s.