Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 antibody (TA378307) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37‚ÑÉ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.